首页|期刊导航|中国农业科学（英文版）|Regulation of Propylene and 1-Methylcyclopropene on Expressions of ACS and ACO Genes in Persimmon Fruits
中国农业科学（英文版）2009，Vol.8Issue(10)：1187-1192,6.DOI:10.1016/S1671-2927(08)60328-4
Regulation of Propylene and 1-Methylcyclopropene on Expressions of ACS and ACO Genes in Persimmon Fruits
Regulation of Propylene and 1-Methylcyclopropene on Expressions of ACS and ACO Genes in Persimmon Fruits
摘要
Abstract
The regulation of postharvest treatment with propylene and 1-MCP on ethylene release rate and expressions of 1-aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) genes in Fuping Janshi persimmon (Diospyros kaki L.) fruit were investigated. Fruits were treated with propylene and 1-MCP, then stored at 20℃, ethylene release rate of the treated fruits was measured at regular intervals and RNA was extracted for Northern blotting analysis. The results suggested that treatment with propylene accelerated the expressions of ACS and ACO genes and then enhanced the ethylene biosynthesis, while treatment with 1-MCP inhibited the expressions of two genes and their ethylene biosynthesis. Furthermore, different effects on expressions caused by treatments with propylene and 1-MCP existed in various fruit tissues, the inhibitory effect on ACS and ACO genes by 1-MCP was the strongest in pericarp, followed by pulp and core tissues, in the area near fruit stalk, the inhibitory effect was the weakest. While the enhanced effect on ACS and ACO genes by propylene increased from pulp, core, and pericarp to the area near fruit stalk. Expression of each member of ACS and ACO families in various tissues was also completely different, in control and propylene treatment, DKACS3 gene just expressed in the area near fruit stalk and did not express in other tissues, but DKACS2 gene expressed in four tissues by treatment with propylene.关键词
persimmon (Diospyros kaki L.)/ propylene/ 1-MCP/ ACS/ ACOKey words
persimmon (Diospyros kaki L.)/ propylene/ 1-MCP/ ACS/ ACO引用本文复制引用
LIU Le,RAO Jing-ping,CHANG Xiao-xiao,YI Shun-chao..Regulation of Propylene and 1-Methylcyclopropene on Expressions of ACS and ACO Genes in Persimmon Fruits[J].中国农业科学（英文版）,2009,8(10):1187-1192,6.基金项目
This work was supported by the National Natural Sci-ence Foundation of China (30170662, 30771756). We thank Prof. Wang Qiaochun in the Northwest A & F University, China, for the useful suggestions on the manuscript. （30170662, 30771756）
