中国农业科学2009，Vol.42Issue(12)：4435-4440,6.DOI:10.3864/j.issn.0578-1752.2009.12.040
宁强矮马生长激素基因(GH)的遗传变异分析
Genetic Variation of Growth Hormone Gene on Ningqiang Pony
摘要
Abstract
[Objective] In order to provide a scientific basis for the origin and dwarfish mechanism of Ningqiang pony,the sequence of GH of different animals and some China horse breeds were studied,[Method] The PCR amplification,sequencing and DNAMAN software were used.[Result]Them were 29 genetic variation,11 loci were found in the coding region.Taking the AA sequence of GH of Mongolia horse as standard,there was 1 amino acid variation in Ningqiang pony.Through clustering analysis based on the sequence of mRNA of GH,the homology was 100%,99.8%,99.7%,between Ningqiang and Debao 67,Baise and Mongolia.[Conclusion]The homology level of GH was higher among Equus caballus,Sus scrofa and dog in present study,and the mutation of G→A affects the growth of Ningqiang pony.关键词
宁强矮马/生长激素基因/序列分析/遗传变异Key words
Ningqiang pony/growth hormone gene/sequence analysis/genetic variation分类
农业科技引用本文复制引用
张涛,路宏朝,陈锐,周建军..宁强矮马生长激素基因(GH)的遗传变异分析[J].中国农业科学,2009,42(12):4435-4440,6.基金项目
陕西省教育厅科研项目(07JK212)、陕西理工学院科研项目(SLGQD0513) （07JK212）
