中国农业科学（英文版）2009，Vol.8Issue(10)：1159-1165,7.DOI:10.1016/S1671-2927(08)60325-9
Study on Haploid Inducing and Its Meiotic Abnormality in Maize
Study on Haploid Inducing and Its Meiotic Abnormality in Maize
摘要
Abstract
The haploid-inducing line Stock 6 was used to produce haploid maize and expected to obtain maize haploid plants successfully. The detailed meiotic studies on selected haploid maize (n=x=10) were conducted. Cytogenetie analysis revealed a high frequency of meiotic abnormality occurred in both meiosis Ⅰ and meiosis Ⅱ. During the prophase I, univalents were common configurations, and there were bivalents or trivalents in some pollen mother cells, however, a few cells containing five bivalents were also observed. After prophase I, chromosomes did not congregate in a single metaphase plate but they were scattered in the cytoplasm. At anaphase I, the chromosome distribution was highly irregular with almost all possible combinations. In some cells, chromosomes were grouped into the three or four masses and several spindles appeared. At the tetrad stage of meiosis Ⅱ, cytokinesis splitting abnormality occurred, and a variety of diad, triad, tetrad, pentad, hexad, as well as decury microspores were easily observed. As a consequence of abnormalities of the two meiotic stages, various microspores and the pollen grains with different size were formed, and its pollen grains were almost completely sterile.关键词
Zea mays L/ haploid maize/ meiosis/ chromosome/ Stock 6Key words
Zea mays L/ haploid maize/ meiosis/ chromosome/ Stock 6引用本文复制引用
TANG Qi-lin,FENG Yun-chao,HAN Xue-li,ZHENG Ming-min,RONG Ting-zhao..Study on Haploid Inducing and Its Meiotic Abnormality in Maize[J].中国农业科学（英文版）,2009,8(10):1159-1165,7.基金项目
The authors wish to thank Maize Genetics COOP Stock Center (USDA of USA) and Liu Jilin (Huazhong Agri-cultural University, Wuhan, China) for providing the Stock 6 line, and E. H. Coe (University of Missouri, Columbia, Mo., USA) for sending his useful papers. This work was partially supported by the National 973 Program of China (2007CB108900), the National Natu-ral Science Foundation of China (30671308), Sichuan Youth Science and Technology Fund, China (2007Q14-032), Ministry of Education Innovation Team Develop-ment Plan, China (IRT0453), and Sichuan Forage Grass Breeding Project, China. （USDA of USA）
