中国水稻科学2009，Vol.23Issue(5)：555-558,4.DOI:10.3969/j.issn.1001-7216.2009.05.17
水稻叶片自然衰老过程中Rubisco大亚基的含量变化
Changes in Relative Content of Rubisco Large Subunit in Naturally Senescing Rice Leaves
摘要
Abstract
A rice mutant with low chlorophyll b and its wild type rice were adopted to investigate the changes in photosynthetic oxygen evolution rate, and content and sensitivity to trypsin proteolysis of Rubisco large subunit (LSU) during leaf senescence, as well as the effects of antioxidant and oxidant on changes in relative content of Rubisco LSU. The results showed that after full expansion of leaves in the mutant and its wild type plants, oxygen evolution and relative content of Rubisco LSU declined significantly. The degradation of Rubisco LSU could be retarded by antioxidants (reduced glutathione and ascorbic acid) but accelerated by oxidants (oxidized glutathione and H_2O_2). The oxygen evolution rate in the mutant was higher than that in the wild type. On the contrary, the degradation of Rubisco LSU was slower in the mutant than in the wild type as leaf senescence. Lower sensitivity to trypsin proteolysis of Rubisco LSU was found in the mutant in comparison with the wild type. It is suggested that reactive oxygen species (ROS) mediate the degradation of Rubisco LSU.关键词
水稻/叶绿素b/突变体/衰老/光合放氧/1,5-二磷酸核酮糖羧化酶/加氧酶Key words
rice/chlorophyll b/mutant/senescence/oxygen evolution/rubulose-1,5-bisphophate carboxylase分类
农业科技引用本文复制引用
李瑞,周玮,李丽,陆巍..水稻叶片自然衰老过程中Rubisco大亚基的含量变化[J].中国水稻科学,2009,23(5):555-558,4.基金项目
南京农业大学科技创新基金资助项目(Y200523). （Y200523）
