中国水稻科学2009，Vol.23Issue(6)：611-615,5.DOI:10.3969/j.issn.1001-7216.2009.06.08
稻瘟病菌致病性增强突变体B11的基因分析
Analysis on Gene Locus of Magnaporthe oryzae B11, a Pathogenicity-Enhanced Mutant
摘要
Abstract
A Magnaporthe or yzae mutant Bll was obtained by Agrobacterium-mediated transformation (AtMT) method, which enhanced the pathogenicity to barley. Southern blotting analysis indicated that T-DNA insert in the Bll genome was single copy. TAIL-PCR and sequence alignment analysis indicated that the T-DNA insertion broke the putative gene locus MG01679. By using PCR-based method, the DNA and cDNA of the gene locus were cloned and sequenced. The open reading frame of the gene includes one intron and two exons. And the coding sequence is 696 bp long and encodes a 231 amino acid peptide. Protein similarity analysis indicated that the product of the gene belongs to the ThiJ/Pfp I protein family, and the gene was thus assigned MgThiJl. MgThiJl shows 57% similarity to FOXG_09029 from Fusarium oxysporum , and 54% similarity to FGSG_08979 from Fusarium graminearum in protein sequence. MgThiJl gene may be a negative regulator to vegetative growth and pathogenicity in filamentous fungi, and its specific mechanism need to be studied further.关键词
稻瘟病菌/突变体/致病性/基因/基因功能/蛋白序列Key words
Magnaporthe oryzae/mutant/pathogenicity/gene/gene function/protein sequence分类
生物科学引用本文复制引用
吴小燕,王艳丽,孙国昌,王教瑜,张震,井金学,杜新法,柴荣耀,毛雪琴,邱海萍,姜华..稻瘟病菌致病性增强突变体B11的基因分析[J].中国水稻科学,2009,23(6):611-615,5.基金项目
浙江省自然科学基金资助项目(Y306638) （Y306638）
浙江省重大科技攻关计划资助项目(2007C12905) （2007C12905）
国家自然科学基金资助项目(30900933, 30970082). （30900933, 30970082）
