中国水稻科学2009，Vol.23Issue(6)：653-656,4.DOI:10.3969/j.issn.1001-7216.2009.06.15
利用功能标记鉴定普通野生稻中的白叶枯病抗性基因
Application of Functional Marker to Identify Genes for Bacterial Blight Resistance in Oryza rufipogon
摘要
Abstract
Field resistance of nine accessions of common wild rice (Oryza rufipogon Griff. ) and a highly susceptible variety IR24 was evaluated with nine strains of Xanthomonas oryzae pv. oryzae from the Philippines. The result showed that IR24 was highly susceptible to all of the strains and six common wild rice materials resisted total nine strains, accounting for 67% of the total. Two materials from Yulin, Guangxi Zhuang Autonomous Region and Wanning, Hainan Province were susceptible to the strains PXO280 and PXO71, respectively, and a material from Gaozhou, Guangdong Province was susceptible to the strains PXO79, PXO99 and PXO339, but they were resistant to the other strains. It is concluded that there was at least one resistance gene in every material. PCR detection for the presence of cloned resistance genes with the functional markers for xa5 , xal3 , Xa21 and Xa27 revealed that there were no xa5 and Xa21 in all of these materials. Four materials contained recessive resistance gene xal3, which were all heterozygous. There were five homozygotes with dominant resistance gene Xa27 and three homozygotes harboring the recessive gene xa27 and one material with neither xa27 nor Xa27.关键词
普通野生稻/白叶枯病/抗性基因/功能标记Key words
Oryza rufipogon/bacterial blight/resistance gene/functional marker分类
农业科技引用本文复制引用
夏志辉,韩飞,高利芬,袁潜华,翟文学,刘迪,罗越华..利用功能标记鉴定普通野生稻中的白叶枯病抗性基因[J].中国水稻科学,2009,23(6):653-656,4.基金项目
海南省重点学科建设资助项目 （）
国家863计划资助项目(2006AA10Z1C8) （2006AA10Z1C8）
中国科学院知识创新工程资助项目(KSCX-YW-N-009-02, KSCX1-YW-03) （KSCX-YW-N-009-02, KSCX1-YW-03）
国家973计划前期研究专项资助项目(2009CB126004) （2009CB126004）
海南省自然科学基金资助项目(309019). （309019）
