中国水稻科学2009，Vol.23Issue(5)：489-494,6.DOI:10.3969/j.issn.1001-7216.2009.05.07
北方杂交粳稻亲本遗传背景分析
Genetic Basis of Parents of japonica Hybrid Rice in the Northern China
摘要
Abstract
To reveal the genetic basis of parents of japonica hybrid rice in the Northern China, genetic differentiation and indica component analysis were conducted on the main parents of japonica hybrid rice and their succeeding materials by using simple sequence repeats(SSR) marker.The genomic DNAs of 96 rice materials were amplified with 26 primers, and a total of 71 alleles were detected, with 2.73 alleles per primer on average. The molecular phylogenetic tree built by UPMGA method showed that the rice materials were divided into 5 groups at the genetic similarity coefficient of 0.66.Relatively great genetic differentiation was found among groups.The extent of indica component value (AD_i) of the 64 restorer lines was from 0.056 to 0.684, and that of 24 sterile lines was from 0.087 to 0.381. It is suggested that the extent of indica component value (AD_i) of a hybrid rice combination with high heterosis should be from 0.20 to 0.40, that of its restorer line should be from 0.26 to 0.50, and that of its sterile line should be below 0.20, and the genetic distance of its parents should vary from 0.30 to 0.60. Besides, the female parent of a hybrid rice combination with high heterosis should have a high yield potential, because the yield of a hybrid rice combination was more affected by its sterile line than its restorer line.关键词
杂交水稻/不育系/恢复系/微卫星标记/遗传差异/籼性程度Key words
hybrid rice/sterile line/restorer line/simple sequence repeats/genetic differentiation/indica component value分类
农业科技引用本文复制引用
王昌华,张燕之,华泽田,徐正进,郑文静,赵家铭,刘欣,王辉,周毓珩..北方杂交粳稻亲本遗传背景分析[J].中国水稻科学,2009,23(5):489-494,6.基金项目
农业部农业科技跨越计划资助项目(2007GB000060) （2007GB000060）
辽宁省稻作研究所青年人才启动专项基金资助项目. （）
