中国神经再生研究（英文版）2009，Vol.4Issue(10)：791-795,5.DOI:10.3969/j.issn.1673-5374.2009.10.012
Recombinant-activated factor Ⅶ and neuronal apoptosis in a rat model of intracerebral hemorrhage
Recombinant-activated factor Ⅶ and neuronal apoptosis in a rat model of intracerebral hemorrhage
Qiang Li 1Wei Li 1Suju Ding 2Jianping Tang 1Jing Fang 1Benqiang Deng 2Tao Wu2
作者信息
- 1. Ninth People's Hospital of Shanghai Jiao Tong University
- 2. Changhai Hospital,Second Miilitary Medical University of Chinese PLA
- 折叠
摘要
Abstract
BACKGROUND:Activated clotting factor Ⅶ has been demonstrated to exhibit obvious anti-apoptosis effects.OBJECTIVE:To observe the effect of activated clotting factor Ⅶ on neuronal apoptosis at different time points following rat intracerebral hemorrhage (ICH).DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Neurobiological Laboratory of Second Military Medical University from October 2005 to April 2006.MATERIALS:Recombinant-activated clotting factor Vlla (rFⅧa) was purchased from Danish Novo Nordisk,Denmark.In situ cell death detection kit-POD kit was purchased from Roche,Switzerland.Caspase-3 activity determination kit from Biovision,USA.METHODS:A total of 72 healthy,male,Sprague Dawley rats,aged 5-8 months,were randomly assigned to three groups (n=24):sham-operated,ICH model,and rFⅧa.In the ICH model and rFⅧa groups,80.0 μL autologous non-clotting blood from rat tails was injected into the right caudate putamen to establish the ICH.The empty microinjector was inserted into the caudate putamen in the sham-operated group.The ICH model and rFⅧa groups were subdivided into four subsets separately:6,24,72 hours and 7 clays following ICH.The rats in the rFⅧa group were injected with 160 μg/kg rFⅧa via the dorsal vein of the penis.MAIN OUTCOME MEASURES:Apoptotic cells were detected in the right caudate putamen by TUNEL;caspase-3 activity by spectrophotometry;and rat neurological function was evaluated by neurological functional impairment scales.RESULTS:Rat neurological function was deteriorated at 24,72 hours,and 7 days following ICH.The TUNEL-positive cells and caspase-3 activity in the right caudate putamen was significantly increased in the ICH rats (P＜0.05);rFVlla treatment reduced the number of TUNEL-positive cells and caspase-3 activity in the right caudate putamen (P＜0.05),and neurological function was significantly improved (P＜0.05).CONCLUSION:rFⅧa was applied within 72 hours after ICH,which reduced the amount of neuronal apoptosis and promoted neurological function restoration by possibly inhibiting caspase-3 activity.关键词
intracerebral hemorrhage/apoptosis/activated clotting factor Ⅷ/caspase-3/in situ nick-end labelingKey words
intracerebral hemorrhage/apoptosis/activated clotting factor Ⅷ/caspase-3/in situ nick-end labeling分类
医药卫生引用本文复制引用
Qiang Li,Wei Li,Suju Ding,Jianping Tang,Jing Fang,Benqiang Deng,Tao Wu..Recombinant-activated factor Ⅶ and neuronal apoptosis in a rat model of intracerebral hemorrhage[J].中国神经再生研究（英文版）,2009,4(10):791-795,5.
