中国输血杂志2009，Vol.22Issue(8)：642-645,4.
特发性血小板减少性紫癜外周血单个核细胞Toll样受体表达研究
Expression of Toll like receptors on peripheral blood mononuclear cells from patients with idiopathic thrombocytopenic purpura
梁晓浏 1王保龙 1上官志敏 1叶书来 1陈家萍 1廖艳秋 1丁凯阳 2完晓菊 1林正明3
作者信息
- 1. 安徽医科大学附属省立医院输血科,安徽,合肥,230001
- 2. 安徽省立医院血液科
- 3. 安徽省合肥市第一人民医院输血科
- 折叠
摘要
Abstract
Objective To study the level of the Toll like-receptor mRNA expression on PBMCs from patients with the idiopathic thrombocytopenic purpura. Methods We performed quantitative real-time reverse transcription-polymerase chain reaction (SYBR Green Ⅰ fluorescent dye combination technique) analyses of PBMCs from 30 idiopathic thrombocytopenic purpura (ITP) patients and 30 healthy subjects, to estimate the relative levels of TLR2,TLR4,TLR6,TLR7 and TLR9 mRNA expression. Results Relative expression levels of TLR2 and TLR7 mRNAs in FTP group ( n = 30) were significantly higher than those in health control group ( n = 30) ( P < 0. 05) , and relative expression levels of TLR4 , TLR6 and TLR9 mRNA showed no significant difference between the two groups( P > 0. 05). Conclusion Expressions of TLR2 and TLR7 mRNA are up-regulated in patients with ITP, which may play an important role in the pathogenesis of ITP.关键词
特发性血小板减少性紫癜/外周血单个核细胞/Toll样受体/RT-PCRKey words
Idiopathic thrombocytopenic purpura/Peripheral blood mononuclear cells/Toll like-receptors/Real-time quantitative RT-PCR分类
临床医学引用本文复制引用
梁晓浏,王保龙,上官志敏,叶书来,陈家萍,廖艳秋,丁凯阳,完晓菊,林正明..特发性血小板减少性紫癜外周血单个核细胞Toll样受体表达研究[J].中国输血杂志,2009,22(8):642-645,4.
