中国输血杂志2009，Vol.22Issue(8)：646-649,4.
改良血小板添加液低温(10℃)保存血小板的动物实验研究
Efficacy of modified PLT additive solution for platelets storage at low temperature (10℃) on rabbit thrombocytopenia model
摘要
Abstract
Objective To evaluate the in vivo efficacy of modified platelet (PLT) additive solution(PAS-ⅢM) with trehalose as a substitute for plasma for the storage of platelet concentrates at low temperature (10℃). Methods Rabbit platelet concentrates were collected and stored in different media and temperature conditions, plasma (22℃), 70% PAS-Ⅲ M/30% plasma (10℃) and plasma/trehalose (-85℃). Platelets in plasma (22℃) on storage day 3, in 70% PAS-Ⅲ M/30% plasma (10℃) on storage day 3, 7, 9, and in plasma/trehalose (- 85℃) thawed on day 20 were transfused to rabbit thrombocytopenia model. The in vivo recoveries and survivals of the three preserved platelets and fresh platelets were measured and evaluated after transfusion into rabit model of thrombocytopenia. Results The survival of platelets stored in 70% PAS-ⅢM/30% plasma(10℃) on day 9 was lower than that of fresh platelets (P<0.05). The recoveries and survivals of platelets stored in plasma (22℃) , in 70% PAS-ⅢM/30% plasma (10℃) on day 3 and day 7 were not different from fresh platelets (22℃) (P >0.05). Recovery and survival of frozen platelets were significant lower than those of the other groups (P <0. 05) . Conclusion PAS-ⅢM with trehalose can protect the chilled rabbit platelets, prolong the circulation of refrigerated rabbit platelets, and could be a good substitute for plasma for platelet storage.关键词
血小板/PAS/低温保存/海藻糖Key words
Rabbit platelet/PAS/Cold-storage/Trehalose分类
临床医学引用本文复制引用
王昕,史蓉华,李静,黎儒青,蒋天伦,赵树铭..改良血小板添加液低温(10℃)保存血小板的动物实验研究[J].中国输血杂志,2009,22(8):646-649,4.基金项目
第三军医大学西南医院临床创新课题(2008002) （2008002）
