中国兽医科学2009，Vol.39Issue(9)：753-758,6.
弓形虫代谢分泌抗原对猪T细胞亚群的影响
Effect of Toxoplasma gondii excreted/secreted antigens on porcine T lymphocyte subgroups
摘要
Abstract
To elucidate the effects of Toxoplasma gondii excreted/secreted antigen(E/SA) on piglet's T lymphocyte subgroups and antibodies,piglets were randomly divided into six groups of E/SA, E/SA+CpG(unemulsification),E/SA+ CpG, E/SA+ IL-2, E/SA + IL-2 + CpG groups and the control group. The dynamic changes of the CD4~+ and CD8~+ T lymphocyte subgroups in the piglet's peripheral blood were determined by flow cytometry, and antibody levels were tested by indirect hemagglutination assay. The results showed that on weeks four post-immunization with T. gondii E/SA,the peripheral blood CD4~+ and CD8~+ T lymphocyte levels and specific antibody levels of the immune groups had significantly increased compared with those of the control group. The specific antibody levels further increased on week 1 after inoculation,the CD4~+ T cells levels and CD4~+ /CD8~+ ratio of the immune groups reduced to varying degrees than two weeks post-infection, CD4~+ T cells levels and CD4~ +/ CD8~+ ratio in the immune group increased to immunized levels,CD8~+ levels declined. So the T. gondii E/SA can improve the percentage of the piglet's CD4~+ and CD8~+ T lymphocytes and the specific antibody levels in peripheral blood.关键词
弓形虫/代谢分泌抗原/T细胞亚群/抗体水平Key words
Toxoplasma gondii/excreted/secreted antigen/T lymphocyte subgroup/antibody level分类
农业科技引用本文复制引用
蔡志杰,王艳华,付宝权,李文卉,芦赟,张燕丽,曹丽艳,张德林..弓形虫代谢分泌抗原对猪T细胞亚群的影响[J].中国兽医科学,2009,39(9):753-758,6.基金项目
国家"十一五"科技支撑计划项目(2007BAD40B05) （2007BAD40B05）
公益性行业(农业)科研专项经费项目(200803017) （农业）
甘肃省重大专项科技项目(2GS063-A43-013) （2GS063-A43-013）
