中国兽医科学2009，Vol.39Issue(9)：764-768,5.
我国旋毛虫地理株基因组DNA多态性的RAPD分析
Analysis of polymorphism in genomic DNA of Trichinella isolates in China by RAPD
摘要
Abstract
Using international reference strains of five Trichinella species[Trichinella spiralis (T1), T. nativa(T2) ,T. britovi(T3) ,T. pseudospiralis(T4) and T. nelsoni(T7)] as control,randomly amplified polymorphic DNA(RAPD) technique was performed to amplify genomic DNA of seven Trichinella isolates from Henan Province, Hubei Province, Yunnan Province, Xi'an of Shaanxi Province, Tianjin Metropolis, and Tongjiang Prefecture and Harbin of Heilongjiang Province. The genetic distance of the isolates was measured by the percentage of unshared bands,and the dendrogram was constructed. The results showed that the genetic distance between T1 and the seven Chinese Trichinella isolates was less than 0. 369 5. The genetic similarity between Harbin and Yunnan isolates and that between Henan and Xi' an isolates was 83. 33% and 86. 35%, respectively. The genetic distance between Tianjin and Harbin or Yunnan isolates was less than 0. 250 0. The genetic distance between Tongjiang isolate and other 6 Chinese isolates was more than 0. 284 1 ,but which between Tongjiang isolate and T1 was less than 0. 263 3. The genetic distance between Hubei isolate and other 6 Chinese isolates was more than 0. 301 2. The genetic distance between T2,T3,T4 or T7 and seven Chinese Trichinella isolates was more than 0. 589 8. All the seven Chinese Trichinella isolates belonged to Tl. Tianjin, Harbin and Yunnan isolates were classified into one group, Henan and Xi'an isolates were classified into another group, and Tongjiang isolate or Hubei isolate was classified into an individual group.关键词
旋毛虫属/中国地理株/基因组多态性/随机扩增多态性DNA/分子鉴定Key words
Trichinella/Chinese isolates/genomic polymorphism /RAPD/ molecular identification分类
农业科技引用本文复制引用
张玺,崔晶,王中全..我国旋毛虫地理株基因组DNA多态性的RAPD分析[J].中国兽医科学,2009,39(9):764-768,5.基金项目
国家自然科学基金项目(30671834) （30671834）
河南省重大公益性科研项目(2008) （2008）
