中国兽医科学2009，Vol.39Issue(9)：779-785,7.
猪圆环病毒2型血清中和抗体阻断ELISA检测方法的建立及应用
Development and application of a blocking ELISA for detection of serum neutralizing antibody against porcine circovirus type 2
摘要
Abstract
A blocking ELISA was developed for detecting porcine serum neutralizing antibody against porcine circovirus type 2(PCV2) based on a neutralizing monoclonal antibody (McAb) to PCV2-Cap pro-tein. There was 96. 0% coincidence rate between the blocking ELISA and the immunoperoxidase monolayer assay based on detection of 353 swine serum samples. The sensitivity and specificity of the blocking ELISA was 97. 9% and 92. 4%, respectively, and there was no cross-reaction with the positive sera infected with porcine circovirus type 1,porcine parvovirus,porcine pseudorabies virus,transmissible gastroenteritis virus of swine,porcine epidemic diarrhea virus,classical fever virus,or porcine reproductive and respiratory syn-drome virus, respectively. The detection results of neutralizing antibody against PCV2 by the blocking ELISA was significant positive correlation(r=0. 997 0) with that by the serum neutralization test(SNT), and the sensitivity of the blocking ELISA was higher than that of SNT. Neutralizing antibodies could be detected from the sera of the swine vaccinated with PCV2 inactivated vaccine after two weeks,all sera was positive after four weeks. 703 serum samples collected from Heilongjiang, Jilin and Liaoning provinces showed 73. 0% positive rates,indicating that PCV2 infection rate in the swine herds in the three provinces was high and the damage caused by PCV2 was serious.关键词
猪圆环病毒2型/中和抗体/单克隆抗体/阻断ELISAKey words
porcine circovirus type 2/neutralizing antibody/monoclonal antibody/blocking ELISA分类
农业科技引用本文复制引用
黄立平,刘长明,危艳武,陆月华,郭龙军..猪圆环病毒2型血清中和抗体阻断ELISA检测方法的建立及应用[J].中国兽医科学,2009,39(9):779-785,7.基金项目
国家科技支撑计划项目(2006BAD06A07) （2006BAD06A07）
国家自然科学基金项目(30871859) （30871859）
