中国兽医科学2009，Vol.39Issue(9)：803-809,7.
光滑型布鲁杆菌抗体竞争ELISA检测方法的建立
Development of a competitive ELISA for detection of antibodies against smooth Brucella
摘要
Abstract
A competitive enzyme-linked immunosorbent assay(cELISA) for detecting serum antibodies against smooth Brucella was developed using Brucella S-LPS C epitope McAbs 16C5 as competitive anti-body and using the purified S-LPS of Brucella vaccine strain M5-90 as coating antigen. Serum samples from cattle (n= 507) were tested by cELISA, the rose Bengal plate test(RBT) and IDEXX indirect enzyme-linked immunosorbent assay (iELISA) Kits, respectively. The coincidence between cELISA and RBT or IDEXX iELISA Kit was 84. 7% or 91. 2% respectively. 142 out of the 507 serum samples were tested by cELISA,Svanova cELISA and iELISA kit provided by Heilongjiang Animal Inspection Institute, and the results showed that the coincidence between cELISA and iELISA or Svanova cELISA Kit was 94. 4% or 97. 2%,respectively. The result by cELISA was in good agreement (κ>0. 5) with that by other method. It was concluded that cELISA with good reliability could provide a valuable tool for the serological diagnosis of brucellosis and could eliminate false-positive serological reactions caused by Yersinia enterocolitica O:9, Escherichia coli O:157 and so on.关键词
布鲁杆菌/竞争酶联免疫吸附试验/单克隆抗体Key words
Brucella/competitive ELISA(cELISA) /monoclonal antibody分类
农业科技引用本文复制引用
王加兰,胡森,高红霞,郑孝辉,步志高..光滑型布鲁杆菌抗体竞争ELISA检测方法的建立[J].中国兽医科学,2009,39(9):803-809,7.基金项目
国家高技术研究发展计划(863)项目(20060102Z449) （863）
哈尔滨市科技攻关项目(2007AA6CN029) （2007AA6CN029）
