中国兽医科学2009，Vol.39Issue(9)：817-820,4.
pIRES2-EGFP-LR真核表达载体的构建及其在293T细胞中的表达
Construction of eukaryotic expression plasmid pIRES2-EGFP-LR and its expression in 293T cells
摘要
Abstract
To explore the physiologic function of laminin receptor (LR) and its role in the development of diseases,a LR gene was amplified by RT-PCR with the total RNA extracted of the cultured cortical neu-rons of SD rats as template. To construct pMD18-T-LR, the amplified gene was cloned into the vector pMD18-T. The LR gene from pMD18-T-LR digested by restriction enzymes Bgl Ⅱ +Sal I was cloned into the eukaryotic expression vector pIRES2-EGFP with the reported gene EGFP (enhanced green fluores-cence protein). The positive recombinant was deisignated with pIRES2-EGFP-LR. After being identified by restriction enzyme digestion and sequencing,the recombinant pIRES2-EGFP-LR was transfected into 293T cells by liposomes. The expression of the recombinant pIRES2-EGFP-LR in the 293T cells was observed by fluorescence microscope and laser scanning confocal microscope. The results showed that the LR of pIRES2-EGFP-LR was successfully expressed in the 293T cells.关键词
层黏蛋白受体/真核表达载体/转染Key words
laminin receptor/eukaryotic expression vector/transfection分类
生物科学引用本文复制引用
宁章勇,罗敏意,亓文宝,李小康,程艳芬..pIRES2-EGFP-LR真核表达载体的构建及其在293T细胞中的表达[J].中国兽医科学,2009,39(9):817-820,4.基金项目
国家自然科学基金项目(30700603) （30700603）
广东省自然科学基金项目(7300744) （7300744）
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723) （IRT0723）
华南农业大学校长基金项目[152(3)] （3）
