中国兽医科学2009，Vol.39Issue(11)：994-998,5.
猪传染性胃肠炎病毒重组N蛋白单克隆抗体的制备及鉴定
Development and identification of monoclonal antibodies against recombinant nucleoprotein of transmissible gastroenteritis virus
摘要
Abstract
The monoclonal antibodies (McAbs) against transmissible gastroenteritis virus (TGEV) N protein were developed by fusing SP2/0 cells with splenocytes of BALB/c mice immunized with the puri-fied recombinant N protein of TGEV. Three hybridoma cells,JY(4)-1,JY(4)-2 and JY(4)-3, were exa-mined by an indirect ELISA and subcloned 4 times by the limited dilution. The titers of 3 hybridoma cells' culture supernatants,murine ascites and sera were 1 : 512,1 : 10~5 or 1 : 10~5 in the indirect ELISA,respec-tively. The inducing ascites method was the best for producing monoclonal antibodies. The 3 McAbs were in strong reactivity with the ST cells infected with TGEV and no cross-reaction was observed between the McAbs and porcine parvovirus,or porcine reproductive and respiratory syndrome virus or porcine rotavirus in the indirect immunofluorescence assay. The three McAbs, being classified into IgG2a, were highly stable.关键词
猪传染性胃肠炎病毒/重组N蛋白/单克隆抗体/杂交瘤Key words
transmissible gastroenteritis virus/ recombinant N protein/ monocional antibody/ hybridoma分类
农业科技引用本文复制引用
黄小波,李娜,文心田,曹三杰,余慧,杨恒,秦学远,尹杰..猪传染性胃肠炎病毒重组N蛋白单克隆抗体的制备及鉴定[J].中国兽医科学,2009,39(11):994-998,5.基金项目
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848) （IRT0848）
四川省教育厅资助项目(08zb075) （08zb075）
