中国兽医科学2009，Vol.39Issue(11)：1014-1017,4.
猪促卵泡激素α和β亚基基因的克隆及其重组腺病毒表达载体的构建
Cloning of α and β subunit genes encoding swine FSH and construction of recombinant adenovirus with α or β subunit gene
李儒曙 1汪涛 2刘宇 3伍时达1
作者信息
- 1. 珠海市动植物防疫监督检验中心,广东,珠海,519000
- 2. 中山大学,生命科学学院,广东,广州,510275
- 3. 华南农业大学,兽医学院,广东,广州,510642
- 折叠
摘要
Abstract
In order to explore the feasibility of artificial preparation of swine FSH,the total RNA was extracted from tissues of swine pituitary gland,and the α and β subunit genes encoding swine FSH were cloned by RT-PCR,and then inserted into the shuttle-CMV plasmid separately to construct recombinant adenovirus plasmids with α or β gene by transforming BJ5183-AD-1 cells. Then, homologous recombination with the α or β gene led AD-293 cells to be CPE,indicating that the recombinant adenovirus with α or β sub-unit gene encoding swine FSH was constructed successfully in vitro.关键词
猪/促卵泡激素/α亚基基因/β亚基基因/克隆/重组腺病毒Key words
swine/ FSH/ α subunit gene/ β subunit gene/ cloning/ recombinant adenovirus分类
农业科技引用本文复制引用
李儒曙,汪涛,刘宇,伍时达..猪促卵泡激素α和β亚基基因的克隆及其重组腺病毒表达载体的构建[J].中国兽医科学,2009,39(11):1014-1017,4.
