中国药房2009，Vol.20Issue(33)：2567-2569,3.
青心酮对小鼠动脉粥样硬化斑块中主要细胞TLR4表达的影响
Effect of 3, 4-Dihydroxyacetophenone(DHAP) on the Expression of TLR4 in Atherosclerotic Plaque of Mice
摘要
Abstract
OBJECTIVE: To explore the effect of 3, 4-Dihydroxyacetophenone (DHAP) on the expression of Toll-like receptor 4 (TLR4) in activated mouse vascular smooth muscle cells (VSMC), endothelial cells (EC) and macrophages (MC).METHODS: Normal thoracic aortas were obtained for primary culture of VSMC and EC and serial subcultivation of RAW264.7 cell lines. The cells were divided into control group, LPS group,simvastatin group, DHAP high dose group and DHAP low dose group. Total mRNA and protein content of each group were analyzed by RT-PCR and Western blot, respectively. RESUL-TS: The expression of TLR4 mRNA and protein content of VSMC, EC and MC in DHAP high dose group were significantly lower than in LPS group(P<0.01).CONCLUSION: DHAP could down-regulate the LPS-activated expression of TLR4 mRNA and protein in VSMC, EC and MC of mice, and TLR4 path might be the anti-inflammatory mechanisms of DHAP.关键词
青心酮/Toll样受体4/动脉粥样硬化Key words
3/4-dihydroxyacetophenone(DHAP)/ Toll-like receptor 4/ Atherosclerosis分类
医药卫生引用本文复制引用
张代娟,刘同美,崔晓栋,刘江月,郭军堂,王建英,叶笃筠..青心酮对小鼠动脉粥样硬化斑块中主要细胞TLR4表达的影响[J].中国药房,2009,20(33):2567-2569,3.基金项目
山东省中医药科技发展计划项目(2005-238) （2005-238）
