中国烟草科学2009，Vol.30Issue(5)：58-61,4.DOI:10.3969/j.issn.1007-5119.2009.05.013
烟草赤星病菌(Alternaria longipes)产角质酶液体发酵条件的优化
Optimal Fermentation Condition of Producing Cutinase by Alternaria longipes
摘要
Abstract
Alternaria longipes is the causal agent of tobacco brown spot, a world-wild tobacco disease. Based on Czapek medium supplemented with CaCO_3 and apple cutin, the liquid fermentation conditions were optimized in order to acquire higher cutinase activity. Optimum culture time, temperature and pH were 8 d, 25 ℃ and 5.5, respectively; and optimum carbon and nitrogen sources were dextrose and maltose instead of NaNO_3. According to this fermentation and orthogonal test, the optimum composition of culture medium included dextrose 3%, casein 1%, Tween-80 0.3%. A 11.0-fold higher cutinase activity was obtained compared to the basal medium and the enzyme activity was enhanced from 15.8 U/mL to 175.2 U/mL.关键词
烟草赤星病菌/角质酶/发酵条件Key words
Alternaria longipes/ cutinase/ fermentation condition分类
农业科技引用本文复制引用
刘辉,刘爱新,梁元存,刘菲,魏芳芳..烟草赤星病菌(Alternaria longipes)产角质酶液体发酵条件的优化[J].中国烟草科学,2009,30(5):58-61,4.基金项目
山东省中青年科学家奖励基金(03BS131) （03BS131）
