中国药房2009，Vol.20Issue(28)：2187-2188,2.
硫普罗宁对酒精性脂肪肝大鼠肝组织细胞色素P_(450)2E1表达的影响
Effect of Tiopronin on Cytochrome P_(450)2E1 Expression in Liver of Rats with Alcoholic Fatty Liver
江承平 1吴碧华1
作者信息
- 1. 川北医学院附属医院,南充市,637007
- 折叠
摘要
Abstract
OBJECTIVE: To explore the effect of tiopronin on the expression of cytoehrome P_(450)2E1 (CYP2E1) in liver of rats with alcoholic fatty liver. METHODS: 30 Wistar rats were randomly divided into 3 groups-group A (blank control group), group B (model group) and group C (tiopronin group). Group B and group C were given 50% alcohol intragastrically to establish alcoholic fatty liver model, while group C were intervened with tiopronin (0.15 g·kg~(-1)·d~(-1) administered at 1 hour after alcohol administration) for 5 consecutive weeks. Then all the rats were sacrificed with AST and ALT levels in serum determined. The CYP2E1 expression was detected by RT- PCR; and the pathologic changes of the liver tissues were observed. RESULTS: In group B compared with group A, serum levels of AST, ALT and CYP2E1 expression were significantly higher (P<0.01); compared with group B, the above indexes were markedly lower in group C (P<0.01 or P<0.05); Histopathological examination shows that liver steatosis of group C was significantly abated as compared with group B. CONCLUSION: The preventive and curative effect of tiopronin on alcoholic fatty liver is possibly achieved by down-regulating CYP2E1 expression in liver.关键词
硫普罗宁/酒精性脂肪肝/大鼠/细胞色素/P_(450)2E1Key words
Tiopronin/ Alcoholic fatty liver/ Rat/ Cytochrome P_(450) 2E1分类
医药卫生引用本文复制引用
江承平,吴碧华..硫普罗宁对酒精性脂肪肝大鼠肝组织细胞色素P_(450)2E1表达的影响[J].中国药房,2009,20(28):2187-2188,2.
