中国药房2009，Vol.20Issue(28)：2191-2193,3.
三氧化二砷对人肝癌细胞生长及其尿型纤溶酶受体表达的体外研究
Effect of Arsenic Trioxide on Human Hepatoma Cell Proliferation and Its uPAR Expression
摘要
Abstract
OBJECTIVE: To investigate the effect of arsenic trioxide (As_2O_3) on human hepatoma cells SMMC-7721 viability and the expression of urokinase plasminogen activator receptor (uPAR). METHODS: SMMC-7721 cells were cultured and treated with As_2O_3 at different concentrations for different time with untreated SMMC-7721 cells (without As_2O_3 treatment) served as control. The proliferation rate of the SMMC-7721 cells was measured by MTT assay; the change of cell cycle was detected by flow cytometry analysis; the effect of As_2O_3 on uPAR mRNA levels was analyzed by real-time fluorescent quantitative polymerase chain reaction. RESULTS: As_2O_3 had significant inhibitory effect on SMMC-7721 proliferation in time-dependent and concentration-dependent manner.As compared with control group, As_2O_3 significantly decreased the proportion of cells in G_0/G_1 phase but increased the cell proportion in G_2/M phase, and it markedly down-regulated the level of uPAR mRNA expression (P<0.01). CONCLUSION: The inhibitory effect of As_2O_3 on the proliferation of SMMC-7721 is possibly due to the down-regulation of uPAR mRNA level expression.关键词
三氧化二砷/肝癌细胞株SMMC-7721/增殖/尿型纤溶酶受体/表达Key words
Arsenic trioxide/ Hepatoma cell SMMC- 7721/ Proliferation/ uPAR/ Expression分类
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周艳,于嘉伟,邬红霞,于志坚..三氧化二砷对人肝癌细胞生长及其尿型纤溶酶受体表达的体外研究[J].中国药房,2009,20(28):2191-2193,3.
