中国药房2009，Vol.20Issue(28)：2194-2196,3.
反应停对荷恶性黑色素瘤小鼠的抑瘤作用研究
The Tumor Inhibitory Effect Study of Thalidomide on Malignant Melanoma Bearing Mice
吴广银 1张明智 2何振2
作者信息
- 1. 河南省人民医院,郑州市,450003
- 2. 郑州大学第一附属医院,郑州市,450052
- 折叠
摘要
Abstract
OBJECTIVE: To study the inhibitory effect of thalidomide on B16 melanoma bearing mice. METHODS: Mice were established into melanoma model and divided into negative control group, thalidomide groups (low, middle, high dose) and cyclophosphamide (CTX) group. After receiving corresponding drugs, the tumor weight and the survival time of the tumor - bearing mice were recorded and the tumor inhibition rate was calculated; the expression of vascular endothelial growth factor (VEGF) of the tumors and microvessel densities (MVD) were measured by immunohistochemical staining, and the expression of VEGF mRNA was tested by reverse transcription - polymerase chain reaction (RT - PCR). RESULTS: In thalidomide groups compared with the negative control group, the tumor weight slightly decreased (P >0.05), the survival time was prolonged (P<0.05), the VEGF and VEGF mRNA expression and MVD were decreased (P<0.05) . CONCLUSION: The tumor- inhibitory effect of thalidomide was not significant, but it may prolong the survival time of melanoma bearing mice possibly by inhibiting the expression of VEGF and reducing the angiogenesis.关键词
反应停/B16黑色素瘤/小鼠/血管内皮生长因子/血管生成Key words
Thalidomide/ B16 melanoma/ Mice/ VEGF/ Angiogenesis分类
医药卫生引用本文复制引用
吴广银,张明智,何振..反应停对荷恶性黑色素瘤小鼠的抑瘤作用研究[J].中国药房,2009,20(28):2194-2196,3.
