中国药房2009，Vol.20Issue(29)：2268-2269,2.
耐亚胺培南铜绿假单胞菌对β-内酰胺类抗菌药物耐药相关基因的研究
β- lactamases- resistant Genes in Imipenem-resistant Pseudomonas Aeruginosa
摘要
Abstract
OBJECTIVE: To screen and analyze the β - lactamases - resistant genes in imipenem - resistant pseudomonas aeruginosa (IMPRPa) . METHODS: β - lactamases - resistant genes including IMP, VIM and OprD_2 in 60 clinically isolated IMPRPa strains were detected by polymerase chain reaction (PCR), and sequence analysis was performed on the PCR amplified products of some of IMP and VIM genes. RESULTS: Of the 60 IMPRPa strains, β - lactamases - resistant positive genes were as follows: IMP gene was positive in 7 strains, VIM gene was positive in 18 strains, and both IMP and VIM genes were positive in 3 strains. Sequencing of the PCR products of IMP and VIM genes were IMP - 1 and VIM - 2 types, and OprD_2 gene deletion was noted in 29 strains. CONCLUSION: OprD_2 protein channel deletion and metal - producing β - lactamases are the chief mechanisms for the resistance of IMPRPa to imipenem.关键词
铜绿假单胞菌/β-内酰胺酶类/抗菌药物/耐药基因Key words
Pseudomonas aeruginosa/ β- lactamases/ Antibacterial drug/ Drug resistance genes分类
医药卫生引用本文复制引用
李丰良,高玉红,侯香萍,刘海云,马美芬..耐亚胺培南铜绿假单胞菌对β-内酰胺类抗菌药物耐药相关基因的研究[J].中国药房,2009,20(29):2268-2269,2.基金项目
云南省卫生科技成果三等奖(007J05-3-007-R1) （007J05-3-007-R1）
