中国医科大学学报2009，Vol.38Issue(10)：770-772,3.
卵泡液中NK细胞及其活化与体外受精结局关系的探讨
Relationship between NK Cells in Follicular Fluid and the Outcome of in vitro Fertilization Treatment
摘要
Abstract
Objective To investigate the proportion of follicular fluid CD56~+ natural killer (NK) cells to the total lymphocytes and the ac-tivated CD56~+ NK cells to the total CD56~+ NK cells. To provide an evidence for improving the clinical pregnancy rate in in vitro fertilization (IVF) treatment by regulating the function of NK cells. Methods Triple color flow cytometry was used to analyze the proportion of CD56~+ NK cells and activated CD56~+ NK cells in mature follicular fluid. The IVF treatment outcome was closely followed up. The relationship be-tween the proportion of CD56~+ NK cells and activated CD56~+ NK cells and the IVF treatment outcome was analyzed statistically. Results The proportion of CD56~+ NK cells and activated CD56* NK cells in mature follicular fluid of women who got pregnancy by IVF treatment was (15.57±3.10)% and (2.63±0.94)% respectively,while the proportion of the women who didn't get pregnancy was (19.12±5.37)% and (4.06±2.08)% respectively,which was significantly higher than the pregnant group(P< 0.05). Conclusion The women with lower propor-tion of CD56~+ NK cells and activated CD56~+ NK cells in mature follicular fluid of is easier to get pregnancy by IVF. An altered NK cells pro-file in follicular fluid could therefore influence fertility in IVF treatment outcome.关键词
体外受精/自然杀伤细胞/卵泡液/流式细胞术Key words
in vitro fertilization/natural killer cell/follicular fluid/triple color flow cytomeny分类
医药卫生引用本文复制引用
王秀霞,张合龙,何丽霞,徐小延,杨大磊..卵泡液中NK细胞及其活化与体外受精结局关系的探讨[J].中国医科大学学报,2009,38(10):770-772,3.基金项目
辽宁省自然科学基金资助项目(2007118) （2007118）
