应用凝集素芯片检测胃正常黏膜上皮细胞与胃癌细胞膜表面糖链表达OA北大核心CSCDCSTPCD
Glycan Profiling on the Cell Membrane of Normal Mucosal Epithelial Cells and Gastric Cancer Cells Detect-ed with Lectin Array
目的 应用凝集素芯片检测胃正常黏膜上皮细胞系GES-1和胃癌细胞系SGC7901、MKN45细胞膜表面糖链表达,并对芯片上捕获的细胞进行初步的免疫荧光研究.方法 用胶原酶Ⅱ消化细胞系,利用芯片上的凝集素位点对糖链的亲和特异性捕获荧光标记的细胞,激光共聚焦扫描仪扫描检测细胞膜表面糖链表达.结果 在大多数凝集素位点上,这几种细胞均显示出荧光信号;在凝集素位点WBA、EEL、MAH-Ⅱ上均未显示荧光信号;其中,与GES-1相比,胃癌细胞系SGC7901…查看全部>>
LI Chun-hui;HE Qun;MA Jing-hong;PAN Zhong-cheng;WANG Tian-jiao;ZHANG Yu-kui;ZHAO Yu-jie
Biochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,ChinaBiochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,ChinaBiochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,ChinaBiochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,ChinaBiochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,ChinaBiochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,ChinaBiochip Center,Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,China
医药卫生
凝集素芯片胃癌糖链表达免疫荧光
lectin arraygastric cancerglycan profilingimmunofluorescence
《中国医科大学学报》 2009 (11)
凝集素芯片的制备及其对细胞膜糖链结构的研究
807-809,813,4
国家自然科学基金资助项目(20672144)
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