中国医科大学学报2009，Vol.38Issue(10)：755-757,3.
小鼠钟状期牙胚中Frizzled蛋白的表达
Expression of Frizzled Protein in Mouse Tooth Germ at Bell Stage
摘要
Abstract
Objective To observe the expression of Frizzled protein in mouse tooth germ at bell stage and explore the possible function of Wnt/Frizzled signal molecules. Methods Mouse embryos at 17 and 19 days of gestation (E17 and E19) as well as mice postnatal day 2 (PN2) were employed in our study. The frozen sections of the first lower molar were made and indirect immunofluorescence technique was carried out on the sections. The location of Frizzled protein was observed under the fluorescence microscope. Results Frizzled protein was expressed weakly in the inner- and outer- dental epithelium at E17. At E19,the positive staining was found to distribute at the summit of both pre-ameloblasts and pre-odontoblasts. At P2,Frizzled protein was expressed strongly at the summit of ameloblasts and odontoblasts. Conclusion Wnt/Frizzled signal molecules may be involved in the differentiation of ameloblasts and odontoblasts.关键词
牙胚/钟状期/Frizzled蛋白/成釉细胞/成牙本质细胞Key words
tooth germ/bell stage/Frizzled protein/ameloblast/odontoblast分类
医药卫生引用本文复制引用
高洁,叶新萍,高妍,刘飞..小鼠钟状期牙胚中Frizzled蛋白的表达[J].中国医科大学学报,2009,38(10):755-757,3.基金项目
辽宁省自然科学基金资助项目(20052086) （20052086）
