中国肿瘤生物治疗杂志2009，Vol.16Issue(5)：442-446,5.DOI:10.3872/j.issn.1007-385X.2009.05.004
黏蛋白1多肽通过small MUC1 蛋白抑制肿瘤细胞生长
MUC1 peptide inhibits tumor cell proliferation by binding small MUC1 protein
摘要
Abstract
Objective:To investigate the inhibitory mechanism of mucin 1 tandem repeats peptide (MUC1 peptide) against the proliferation of tumor cells. Methods: Jurkat, Raji, U937, MCF-7, SMMC7721, activated T and RAW264.7 cells were co-cultured with MUC1 peptide; the inhibitory effects of MUC1 peptide on these cells were observed. Jurkat cell-inoculated BABL/c mouse model was established by s.c. injection of Jurkat cells, and then the tumor-bearing mice were treated with MUC1 peptide. The protein interacting with MUC1 peptide was identified by GST pull-down assay. Results: MUC1 peptide inhibited the proliferation of Jurkat, Raji, U937, MCF-7 and SMMC7721 cells, but had no measurable inhibitory effect on activated T cells and RAW264.7 cells. MUC1 peptide significantly inhibited the growth of implanted Jurkat tumors in BABL/c mice (P<0.05). The protein interacting with MUC1 peptide in the lysates of Jurkat and MCF-7 cells was confirmed by GST pull-down assay, with its molecular weight being approximately 115 000. The protein could bind specifically to anti-MUC1 tandem repeat antibodies (GP1.4 and HMPV) and anti-MUC1 cytoplasmic domain antibody (Ab5), indicating it might be a novel isotype of MUC1, and it was named small MUC1 (sMUC1). Conclusion: MUC1 peptide can transduce growth inhibitory signal by interacting with small MUC1 on the surface of tumor cells.关键词
黏蛋白1(MUC1)/small MUC1/肿瘤细胞/生长抑制Key words
mucin 1 (MUC1)/ small MUC1 (sMUC1)/ tumor cell/ growth inhibition分类
医药卫生引用本文复制引用
马吉春,台桂香,赵小霞,方芳,张庆勇,窦蕊,陈文博,柳忠辉..黏蛋白1多肽通过small MUC1 蛋白抑制肿瘤细胞生长[J].中国肿瘤生物治疗杂志,2009,16(5):442-446,5.基金项目
国家自然科学基金资助项目(No.30972782) （No.30972782）
吉林省科技发展计划项目(No. 20080931).Supported by the National Natural Science Foundation of China (No.30972782) （No. 20080931）
the Science and Technology Development Program of Jilin Province (No. 20080931) （No. 20080931）
