中国肿瘤生物治疗杂志2009，Vol.16Issue(5)：458-463,6.DOI:10.3872/j.issn.1007-385X.2009.05.007
TGF-β不敏感前列腺癌特异性CTLs的制备及其抗癌作用
Preparation of TGF-β insensitive cytotoxic T lymphocyte against prostate cancer and its anti-tumor activity
摘要
Abstract
Objective: To prepare TGF-β insensitive cytotoxic T lymphocytes（CTLs）against prostate cancer and to observe its therapeutic effect against prostate cancer. Methods: Peripheral blood mononuclear cells were obtained from prostate cancer patients, and were induced to differentiate into dendritic cells in vitro. Prostate cancer antigen was prepared from the same patients, and tumor antigen specific CTLs were induced by prostate tumor antigen impulsed-DCs. The tumor antigen specific CTLs were infected with lentivirus containing TβRⅡDNglytk gene, and the response of TβRⅡDNglytk transfected-CTLs to TGF-β was examined by Western blotting analysis. Prostate cancer cell-inoculated mice were treated with TGF-β sensitive and insensitive-CTLs separately, and the therapeutic results were compared. Results: CTLs induced by prostate tumor antigen impulsed-DCs grew significantly faster than those induced by non-impulsed-DCs (P<001). TβRⅡDNglytk gene infection reduced the sensitivity of CTLs to TGF-β. The reaction of CTLs infected by lentiviral vector to TGF-β was significantly weaker than that of non-infected CTLs. TGF-β insensitive CTLs significantly inhibited the growth of implanted prostate tumors in mice. Conclusion: TGF-β insensitive prostate tumor antigen specific CTLs have apparent therapeutic effect against prostate cancer, which paves a way for immunotherapy of prostate cancer.关键词
前列腺肿瘤/TGF-β/慢病毒/CTLs/树突状细胞Key words
prostate neoplasms/ TGF-β/ lentivirus/ CTL/ dendritic cell分类
医药卫生引用本文复制引用
杨阔,徐勇,曹现祥,张婷,于明杰,畅继武..TGF-β不敏感前列腺癌特异性CTLs的制备及其抗癌作用[J].中国肿瘤生物治疗杂志,2009,16(5):458-463,6.基金项目
天津市科技计划资助项目(No. 07ZCGYSF01000).Supported by the Science and Technology Project of Tianjin(No. 07ZCGYSF01000) （No. 07ZCGYSF01000）
