慢病毒介导shRNA特异性沉默livin基因促进SPC-A1细胞凋亡OA北大核心CSCDCSTPCD
Lentivirus-mediated shRNA silencing of livin gene promotes apoptosis of SPC-A1 cells
目的:建立慢病毒介导的livin基因沉默系统,探讨其对肺癌细胞凋亡的影响.方法:Livin shRNA慢病毒感染肺腺癌细胞株SPC-A1沉默livin基因表达.应用PI染色经荧光镜下观察SPC-A1细胞凋亡形态,流式细胞术检测SPC-A1细胞凋亡率及亚二倍体峰形成,Real-time PCR及Western blotting方法检测livin和caspase 3表达的改变.结果:livin基因在肺腺癌细胞株SPC-A1中持续高表达.经慢病毒介导s…查看全部>>
Objective:To construct a lentiviral livin shRNA vector to silence livin gene expression, and to study its effect on apoptosis of lung carcinoma cells. Methods: Livin expression in human lung adenocarcina SPC-A1 cells was silenced by lentiviral livin shRNA infection. The morphology of apoptotic cells was observed by propidine iodide staining and fluoroscope; apoptosis rate and sub-lipliod apoptotic peak of SPC-A1 cells were assessed by flow cytometry; express…查看全部>>
陈愉生;李鸿茹;林明;陈刚;谢宝松;许能銮;林立芳
福建医科大学省立临床医学院,呼吸科,福州,350001福建医科大学省立临床医学院,呼吸科,福州,350001福建医科大学省立临床医学院,呼吸科,福州,350001福建医科大学省立临床医学院,呼吸科,福州,350001福建医科大学省立临床医学院,呼吸科,福州,350001福建医科大学省立临床医学院,呼吸科,福州,350001福建医科大学省立临床医学院,呼吸科,福州,350001
医药卫生
肺肿瘤livin基因RNA干扰慢病毒载体细胞凋亡
lung neoplasms livin gene RNA interference lentiviral vector cell apoptosis
《中国肿瘤生物治疗杂志》 2009 (5)
469-473,5
福建省自然科学基金资助项目(No. 2008J0074 ).Supported by the Natural Science Foundation of Fujian Province(No. 2008J0074 )
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