中国肿瘤生物治疗杂志2009，Vol.16Issue(5)：479-483,5.DOI:10.3872/j.issn.1007-385X.2009.05.011
靶向XIAP的siRNA抑制结肠癌LoVo细胞体内外的增殖
XIAP-targeting siRNA inhibits proliferation of colorectal cancer cells in vitro and in vivo
杜利力 1韩春山 1于晓丽 1程晓峰2
作者信息
- 1. 青岛市商业医院,肿瘤内科,山东,青岛,266001
- 2. 青岛市立医院,神经外科,山东,青岛,266071
- 折叠
摘要
Abstract
Objective:To investigate the effect of XIAP (X-linked inhibitor of apoptosis protein)-targeting siRNA on the proliferation of LoVo colorectal cancer cells in vitro and in vivo. Methods: The XIAP-targeting siRNA pSil2.1-shXIAP1 and pSil2.1-shXIAP2 eukaryotic vectors were constructed and were transfected into LoVo cells using Lipofectamine; the stable transfectants LoVo-shXIAP2 were selected by G418. The expressions of XIAP mRNA and protein were determined by RT-PCR and Western blotting. The proliferation of LoVo cells was determined by MTT and colony formation assay. Cell apoptosis was examined by FCM. The influence of XIAP knockdown on the proliferation of LoVo cells in vivo was observed in LoVo-bearing nude mice. Results: The expressions of XIAP mRNA and protein in LoVo-shXIAP2 cells were significantly down-regulated in LoVo-shXIAP2 cells. Compared with un-transfected LoVo cells, the proliferation and colony formation abilities of LoVo-shXIAP2 cells were significantly inhibited (P<0.05); the apoptosis rate of LoVo-shXIAP2 cells was significantly increased (P<0.05). The expression of XIAP protein in LoVo-shXIAP2 implanted tumors was down-regulated and the growth of tumors was significantly inhibited (all P<0.05). Conclusion: pSil2.1-shXIAP2 plasmid can down-regulate the expression of XIAP in LoVo cells and inhibit proliferation of LoVo cells in vitro and in vivo, making XIAP-targeting siRNA a potential new agent in immune therapy of colorectal cancer.关键词
结肠癌/RNA干扰/X连锁凋亡抑制蛋白/增殖/凋亡Key words
colorectal cancer/ RNA interference/ XIAP/ proliferation/ apoptosis分类
医药卫生引用本文复制引用
杜利力,韩春山,于晓丽,程晓峰..靶向XIAP的siRNA抑制结肠癌LoVo细胞体内外的增殖[J].中国肿瘤生物治疗杂志,2009,16(5):479-483,5.
