首页|期刊导航|中华医学杂志（英文版）|Expression of matrix metalloproteinase-1 mRNA in peripheral blood mononuclear cells of systemic lupus erythematosus patients and its relationship with atherosclerosis
Expression of matrix metalloproteinase-1 mRNA in peripheral blood mononuclear cells of systemic lupus erythematosus patients and its relationship with atherosclerosis
ZHANG Hai-ying BAO Shu-meng SHOU Wei-ling LUAN Hai-xia ZHANG Yang FENG Xue TONG Da-wei ZHANG Shu-lan HU Chao-jun ZENG Xiao-feng LI Yong-zhe
中华医学杂志（英文版）Issue(21)：2593-2597,5.
中华医学杂志（英文版）Issue(21)：2593-2597,5.DOI:10.3760/cma.j.issn.0366-6999.2009.21.012
Expression of matrix metalloproteinase-1 mRNA in peripheral blood mononuclear cells of systemic lupus erythematosus patients and its relationship with atherosclerosis
Expression of matrix metalloproteinase-1 mRNA in peripheral blood mononuclear cells of systemic lupus erythematosus patients and its relationship with atherosclerosis
摘要
Abstract
Background Matrix metalloproteinase-1(MMP-1)plays an important role in atherosclerosis.This study was to examine expression of MMP-1 mRNA in peripheral blood mononuclear cells(PBMCs)of patients with systemic lupus erythematosus(SLE),and to explore its relationship with atherosclerosis in SLE.Methods Fluorescent quantitative reverse transcription polymerase chain reaction(RT-PCR)was used to examine the expression of MMP-1 mRNA in PBMCs in 80 SLE patients,including 39 prone to atherosclerosis(Group A)and 41 unprone to atherosclerosis(Group B).Meanwhile,30 patients who were free of cardiovascular diseases and 30 healthy individuals were selected as disease and normal control group(Groups C and D).The changes of MMP-1 gene expression were analyzed by differences of cycle threshold(△Ct),with the following formula:△Ct = Ct_(target) gene-Ct_(reference) gene.Results The expression level of MMP-1 mRNA in Group A was significantly higher than that of group B(△Ct=8.64±2.43 vs △Ct=12.09±2.26,t=6.588,P＜0.01).The expression level of MMP-1 mRNA of SLE patients was significantly higher than that of Group C(△Ct=10.41±2.90 vs △Ct=12.29±2.51,t=-3.135,P＜0.01)and Group D(△Ct=10.41±2.90 vs △Ct=12.48±1.69,t=3.675,P＜0.01).Conclusions In comparison to disease and control group,expression of MMP-1 mRNA in PBMCs of SLE patients was significantly elevated,and significant difference of MMP-1 mRNA expression was also found between SLE patients prone and unprone to atherosclerosis,indicating that expression of MMP-1 mRNA may be correlated with the pathogenesis and activity of atherosclerosis in SLE.关键词
systemic lupus erythematosus/atherosclerosis/matrix metalloproteinases-1/reverse transcription polymerase chain reactionKey words
systemic lupus erythematosus/atherosclerosis/matrix metalloproteinases-1/reverse transcription polymerase chain reaction分类
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ZHANG Hai-ying,BAO Shu-meng,SHOU Wei-ling,LUAN Hai-xia,ZHANG Yang,FENG Xue,TONG Da-wei,ZHANG Shu-lan,HU Chao-jun,ZENG Xiao-feng,LI Yong-zhe..Expression of matrix metalloproteinase-1 mRNA in peripheral blood mononuclear cells of systemic lupus erythematosus patients and its relationship with atherosclerosis[J].中华医学杂志（英文版）,2009,(21):2593-2597,5.基金项目
This work was supported by the grants from the National Natural Science Foundation of China(No.30640084,30471617,30872331)and National Science Technology Pillar Program in the Eleventh Five-year Plan(No.2008BAI59B02). （No.30640084,30471617,30872331）
