首页|期刊导航|中华医学杂志（英文版）|Penetrability of interleukin-1β and its effect on the concentration of tumor necrosis factor-α and interleukin-6 in the aqueous humor of rabbits treated with interleukin-1β
Penetrability of interleukin-1β and its effect on the concentration of tumor necrosis factor-α and interleukin-6 in the aqueous humor of rabbits treated with interleukin-1β
PENG Xiao-yan CHEN Feng-hua WANG Ning-li MA Jian-min Ping-yu Lee LIU Ke-gao Steven M Podos
中华医学杂志（英文版）2009，Vol.122Issue(18)：2165-2168,4.
中华医学杂志（英文版）2009，Vol.122Issue(18)：2165-2168,4.DOI:10.3760/cma.j.issn.0366-6999.2009.18.017
Penetrability of interleukin-1β and its effect on the concentration of tumor necrosis factor-α and interleukin-6 in the aqueous humor of rabbits treated with interleukin-1β
Penetrability of interleukin-1β and its effect on the concentration of tumor necrosis factor-α and interleukin-6 in the aqueous humor of rabbits treated with interleukin-1β
摘要
Abstract
Background Interleukin (IL)-1β may effectively decrease introcular pressure (IOP) when administered by subconjunctival injection in normal rabbit. However, IL-1β is a large molecular agent and an inflammation factor. The aim of this study was to evaluate the penetrability of IL-1β, and the concentrations of both tumor necrosis factor (TNF)-α and IL-6 in the aqueous humor of normal rabbits treated with IL-1β.Methods A total of 170 rabbits were used in the study and were assigned to several different treatment groups as follows: 125 of the rabbits were assigned to two groups. In one group, 33 rabbits were injected subconjunctivally with IL-1β and 39 were injected with saline alone. In the other group, 27 rabbits were given eye drops containing IL-1β (400 ng/ml) and 26 were given saline alone. Aqueous humor (AH) was drawn and the concentration of IL-1β within the fluid measured. The IOP was measured in another six rabbits after administration of eye drops containing IL-1β (400 ng/ml). A further 20 rabbits were assigned to 3 groups as follows: eight untreated normal controls; six injected subconjunctivally with IL-1β; and six injected subconjunctivally with saline alone. AH was drawn and the concentration of TNF-α in the fluid was measured. Another 19 rabbits were assigned to 3 groups as follows: seven untreated normal controls; and six injected subconjunctivally with IL-1β; and six injected subconjunctivally with saline alone. AH was drawn and the concentration of IL-6 in the fluid measured. Measurement of cytokine concentration was by radio-immunoassay in all cases.Results The IL-1β concentration in the AH was higher in those animals in which it had been administered subconjunctivally (P <0.01). The IL-1β concentration in the AH of the animals given eye drops was almost the same as that in the controls (P >0.05). The administration of IL-1β in the form of eye drops had little effect upon lOP reduction. Lower TNF-α concentrations were seen in the AH after the subconjunctival administration of IL-1β, but the concentration of IL-6 was the same as in the normal controls.Conclusions IL-1β shows good corneal penetrability after subconjunctival injection into normal rabbit eyes. The IOP reduction induced by IL-1β is unlikely be associated with an inflammatory response.关键词
interleukin-1β/ tumor necrosis factor-α/ intraocular pressure/ rabbitKey words
interleukin-1β/ tumor necrosis factor-α/ intraocular pressure/ rabbit引用本文复制引用
PENG Xiao-yan,CHEN Feng-hua,WANG Ning-li,MA Jian-min,Ping-yu Lee,LIU Ke-gao,Steven M Podos..Penetrability of interleukin-1β and its effect on the concentration of tumor necrosis factor-α and interleukin-6 in the aqueous humor of rabbits treated with interleukin-1β[J].中华医学杂志（英文版）,2009,122(18):2165-2168,4.基金项目
This study was supported by a grant from the National NaturalScience Foundation of China (No. 30171000). （No. 30171000）
