首页|期刊导航|中华医学杂志（英文版）|Purification and characterization of anti-clotting protein component (ACPF-7221) from venom of Agkistrodon acutus
中华医学杂志（英文版）2009，Vol.122Issue(18)：2169-2173,5.DOI:10.3760/cma.j.issn.0366-6999.2009.18.018
Purification and characterization of anti-clotting protein component (ACPF-7221) from venom of Agkistrodon acutus
Purification and characterization of anti-clotting protein component (ACPF-7221) from venom of Agkistrodon acutus
摘要
Abstract
Background Snake venom contains a number of components with different pharmacological and biological activities, especially in cancer therapy, and has increasingly become a research focus. This study was designed to isolate and purify a novel anti-clotting protein component from the venom of Agkistrodon acutus, and to explore its physico-chemical properties and biological activity.Methods The venom of Agkistrodon was isolated and purified by ion-exchange chromatography on diethylaminoethyl (DEAE)-Sepharose Fast Flow, molecular sieve filtration through Sephadex G75, SP-Sepharose Fast Flow and molecular sieve filtration through Sephadex G50. We detected the activated partial thromboplastin time (APTT) of the eluant to select the anti-clotting protein component of interest. The molecular weight was determined by sodium dodecyl sulfate-polyacrylamid gel electrphoresis (SDS-PAGE) and liquid chromatography. Its protein content was detected by bicinchoninic acid (BCA).Results SDS-PAGE vertical gel electrophoresis showed that the anticoagulant factor is a tripolymer composed of three proteins whose molecular weights are 25 KDa, 30 KDa and 50 KDa. The factor contains about 65% percent protein.Conclusions A novel anti-clotting protein component was purified by ion-exchange chromatography and molecular sieve filtration from the venom of Agkistrodon acutus and was found to be composed of three kinds of proteins.关键词
Agkistrodon acutus/ anticoagulation/ purification/ high performance liquid chromatographyKey words
Agkistrodon acutus/ anticoagulation/ purification/ high performance liquid chromatography引用本文复制引用
RUI Jing,HUAI Jian-guo,ZHANG Ye,CHENG Dong-yun,PAN Xue-bing..Purification and characterization of anti-clotting protein component (ACPF-7221) from venom of Agkistrodon acutus[J].中华医学杂志（英文版）,2009,122(18):2169-2173,5.基金项目
This study was supported by a grant from the Scientific Research Foundation of the Anhui Committee of Education (No. KJ2007A039). （No. KJ2007A039）
