首页|期刊导航|中华医学杂志（英文版）|Protective effect of chrysoeriol against doxorubicin-induced cardiotoxicity in vitro
中华医学杂志（英文版）Issue(21)：2652-2656,5.DOI:10.3760/cma.j.issn.0366-6999.2009.21.024
Protective effect of chrysoeriol against doxorubicin-induced cardiotoxicity in vitro
Protective effect of chrysoeriol against doxorubicin-induced cardiotoxicity in vitro
摘要
Abstract
Background The use of doxorubicin(DOX)is limited by its dose-dependent cardiotoxicity.Reactive oxygen species (ROSs)play an important role in the pathological process of DOX-induced cardiotoxicity.The aim of this study was to evaluate the protective effect of chrysoeriol,a flavone compound,against DOX-induced apoptosis and death in H9c2 cells and to find out its preliminary mechanism.Methods We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)assay,Hoechst33258 staining and measurement of lactate dehydrogenase(LDH)release to evaluate the protective effect of chrysoeriol against DOX-induced apoptosis and death in H9c2 cells.To find out the mechanism of this protective effect,we observed the immunofluorescence of intracellular ROS and measured the activities of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GPx).Furthermore,we evaluated the effect of chrysoeriol on the antitumor activity of DOX in HeLa cells with MTI assay.Results The results of MTT assay,Hoechst 33258 staining and measurement of LDH release showed that chrysoeriol significantly reduced doxorubicin-induced apoptosis and cell death.Chrysoeriol at a dose of 20 pg/ml notably reduced intracellular ROS,decreased the concentration of MDA in the supernatant of DOX-treated H9c2 cells and increased SOD and GPx activities to their normal levels.Further study showed that the addition of chrysoeriol did not affect the antitumor activity of DOX.关键词
chrysoeriol/doxorubicin/cardiotoxicity/reactive oxygen speciesKey words
chrysoeriol/doxorubicin/cardiotoxicity/reactive oxygen species分类
医药卫生引用本文复制引用
LIU Zhe,SONG Xiao-dong,XIN Ying,WANG Xiao-jian,YU Hui,BAI Yong-yi,LIU Jun-hao,ZHANG Chan-na,HUI Ru-tai..Protective effect of chrysoeriol against doxorubicin-induced cardiotoxicity in vitro[J].中华医学杂志（英文版）,2009,(21):2652-2656,5.基金项目
This work was supported by grants from the Ministry of Science and Technology,China(No.2006DFA31500)and Natural Science Foundation of China(No.30640080). （No.2006DFA31500）
