浙江大学学报（理学版）2009，Vol.36Issue(6)：714-719,6.DOI:10.3785/j.issn.1008-9497.2009.06.025
一株嗜盐严格厌氧细菌S191的分离与系统发育分析
Isolation and phylogenetic analysis of halophilic, strictly anaerobic eubacteria S191
石孔兰 1朱丽娴 1吴敏 1朱旭芬1
作者信息
- 1. 浙江大学,生命科学学院,浙江,杭州,310058
- 折叠
摘要
Abstract
Moderately halophilic,strictly anaerobic eubactena SI91 was isolated and purified from the sediment in Northeast Pacific deep-sea nodule province by using Hungate roll-tube technique,the 16S rDNA encoding gene of S191 was proliferated by PCR.the sequence was determined,and homology analysis was completed by "Clustalx" and "Mega" software,a phylogenetic tree of Halanaerobium was constructed,SI91 is considered to be a new member of Halanaerobium.It is a Gram-negative,rod-shaped,non-motile,non-spore-forming obligate anaerobe that owns a cell size of 0.3-0.6 μm× 1.8-3.0 μm.Growth was observed in the presence of 5% -5% NaCl,the optimum salt concentration for growth is 7.5 % -10 %.It grows at pH 6.0-8.9 and the optimum pH for growth is 7.8-8.0.It grows well at temperature 15-42 ℃,and the optimum temperature for growth is 32-37 ℃.The doubling time is about 3.2 h when grown at optimal condition.关键词
严格厌氧细菌/嗜盐/深海沉积物/16S rDNA/系统发育Key words
strict anaerobes halophilic/deep-sea sediment/16S rDNA/phylogenesis分类
生物科学引用本文复制引用
石孔兰,朱丽娴,吴敏,朱旭芬..一株嗜盐严格厌氧细菌S191的分离与系统发育分析[J].浙江大学学报（理学版）,2009,36(6):714-719,6.
