肿瘤防治研究2009，Vol.36Issue(11)：901-904,4.DOI:10.3971/j.issn.1000-8578.2009.11.001
酪氨酸磷酸酶PRL-3单抗的制备及特性鉴定
Preparation and Characterization of Monclonal Antibody against Protein Tyrosine Phosphatase PRL-3
摘要
Abstract
Objective To prepare and identify specific Phosphatase of Regenerating Liver-3 (PRL-3) mon-oclonal antibodies for clinical detection and for further therapeutic intervention. Methods Hybridoma technology was used to prepare PRL-3 monocloual antibodies (MAbs), and the specificities of MAbs a-gainst PRL-3 were evaluated by Western blot and immunoprecipation. Six truncations of PRL-3 were cloned and expressed in prokaryotic cell for identifying the approximate epitope. The binding abilities of MAbs were analyzed by Western blot. Results Among 9 hybridoma clones obtained, 6 (9D8, 9E2,9F4, 11B2, 4D3 and 4D10)could specifically bind to PRL-3, and 4 could react to PRL-3 protein in eu-karyotic cells. Clone 9138,9E2,9F4 and 11B2. cloud bind to the COOH-terrninal of PRL-3, and clone 4D3 and 4D10 cloud bind to 69～95 amino acids. Conclusion MAbs 9D8, 9E2, 9F4, 11B2, 4D3 and 4D10 can react only to PRL-3, which provides potential applications in clinical diagnosis and in future study.关键词
磷酸酶/PRL-3/单克隆/抗体Key words
Phosphatases/ PRL-3/ Monoclonal/ Antibodies分类
医药卫生引用本文复制引用
王莉,杨艳丽,宋子博,廖世秀,黄飞飞..酪氨酸磷酸酶PRL-3单抗的制备及特性鉴定[J].肿瘤防治研究,2009,36(11):901-904,4.基金项目
河南省医学科技创新人才工程资助项目(200585) （200585）
