质谱学报2009，Vol.30Issue(6)：321-326,6.
柱后辅助有机溶剂喷雾对液相色谱-电喷雾-质谱分析的影响
Effect of Auxiliary Electrospray on Liquid Chromatography-Electrospray Ionization-Mass Spectrometry
陈明 1王立群 1杲修杰 1弓景波 1钱小红 2钱令嘉1
作者信息
- 1. 军事医学科学院卫生学环境医学研究所,天津,300050
- 2. 军事医学科学院放射与辐射医学研究所,北京,100850
- 折叠
摘要
Abstract
The efficiency of electrospray ionization was affected by the component of solvent.The effect of different concentration of ACN on mass spectrometry for MRFA, Reserpine,MLtramark1621 and peptide mixture were observed, and found that all of the chemicals have the max mass spectra signal intensity in 70% ACN. Then, the peptide mixtures from myoglobin were analyzed using CapLC-ESI-LTQ system, which were equipped with auxiliary electrospray by 100% ACN, 0. 1% FA. This results show that the detection ratio of peptides and the recovery of protein sequence are remarkable improved.关键词
电喷雾离子化/肽段/质谱分析Key words
electrospray ionization.(ESI)/ peptide/ mass spectrometry分类
化学化工引用本文复制引用
陈明,王立群,杲修杰,弓景波,钱小红,钱令嘉..柱后辅助有机溶剂喷雾对液相色谱-电喷雾-质谱分析的影响[J].质谱学报,2009,30(6):321-326,6.
