质谱学报2009，Vol.30Issue(6)：346-351,6.
液相色谱-串联质谱法测定人血浆中克林霉素
Determination of Clindamycin in Human Plasma by LC-MS/MS
张丹 1韩静 1王涛 1王振龙 1郭丽 1刘会臣1
作者信息
- 1. 北京大学航天临床医学院临床药理室,北京,100049
- 折叠
摘要
Abstract
A LC-MS/MS method for determination of clindamycin in human plasma was developed. After protein precipitation with acetonitrile, the analyte and internal standard (I. S. ), diphenhydramine, were separated on a Shim-pack VP-ODS analytical column using the mobile phase of V(methanol) : V(10 mmol · L~(-1) ammonium acetate (containing 0.25 % formic acid)) =55 : 45 at a flow rate of 0.4 mL · min~(-1). Detection was carried out by electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode. The MRM transitions of m/z 425.2→126.3 and m/z 256.2→167.3 were used to quantify clindamycin and I. S. , respectively. Clindamycin and I. S. are eluted at 1.77 min and 1.79 min, respectively. The calibration curve is linear over the concentration range of 0. 030 0-10.0 mg · L~(-1) with the lower limit of quantitation (LLOQ) 0.030 0 mg · L~(-1). Inter- and intra-day relative standard deviations are both less than 6%, and the relative errors are within ±6%. The mean extract recoveries are (101.1 ± 2.6)%. In the stability studies, clindamycin in plasma is found to be stable under various storage conditions. It is a rapid, sensitive, selective and reliable method for the determination of clindamycin in human plasma. The method is successfully applied to a pharmacokinetic study in healthy volunteers after oral administration of 300 mg clindamycin.关键词
克林霉素/人血浆/液相色谱-串联质谱联用法/药代动力学Key words
clindamycin/ human plasma/ LC-MS/MS/ pharmacokinetics分类
化学化工引用本文复制引用
张丹,韩静,王涛,王振龙,郭丽,刘会臣..液相色谱-串联质谱法测定人血浆中克林霉素[J].质谱学报,2009,30(6):346-351,6.
