作物学报2009，Vol.35Issue(11)：2000-2007,8.DOI:10.3724/SP.J.1006.2009.02000
水稻广亲和基因S5-n的功能标记开发及其应用
Development and Application of a Functional Marker for Wide Compatibility Gene S5-n of Rice
摘要
Abstract
Wide compatibility varieties (WCVs) with SS-n are able to produce highly fertile hybrids when crossed to both indica and japonica varieties. The conventional breeding strategy of pyramiding S5-n is to test the fertility of F1 hybrid. However, this method is labour-consuming, time-consuming and difficult in operation. The technically simplified approach of marker-assisted selection(MAS) has directly and practically used in rice breeding. Therefore, it is urgent to find a convenient and efficient way in WCV breeding with MAS. The wide compatibility gene (S5-n) has 136 bp deletion compared with the indica and japonica S-5 alleles. In order to improve the precision of marker-assisted selection for S5-n, we developed the functional marker S5136 based on the deletion in the DNA sequence of S5-n. The differentiation for S5-n, S5-i, and S5-j in 24 accessions of weedy rice from the district of Yangtze and Huaihe Rivers, 27 accessions of common wild rice (O. rufipogon Griff.) from China, 554 cultivars and genetic resources (O. sativa L.) and 20 breeding lines was investigated by S5136. The results indicated that this marker was low-cost, robust technique that can be utilized to identify the S5-n in WCVs like 02428, Dular and CPSLO17. The S5136 could distinguish the homozygous S5-n/S5-n, homozygous S5-i/S5-i, and heterozygous SS-n/S5-i individuals in F2 population derived from 3037 and 02428. From the tested materials, 13 germplasms with SS-n were identified and conformed by sequencing the S5136 PCR products, and 2 restore lines with S5-n developed from the offspring of 02428 were identified. Also, the compatibility of Kasalath was tested with some indica-japonica hybrids. Therefore, S5136 is a powerful molecular marker in S5-n germplasm screening, perfect marker- assisted selection breeding and seed purity testing for the two line hybrids with Pei'ai 64S as maternal parent.关键词
广亲和基因S5-n/功能标记/亚洲栽培稻/普通野生稻/杂草稻Key words
Wide compatibility gene SS-n/Functional marker/Oryza sativa L./O.rufipogen Griff./O.sativa f.spontanea分类
农业科技引用本文复制引用
杨杰,王军,曹卿,陈志德,仲维功..水稻广亲和基因S5-n的功能标记开发及其应用[J].作物学报,2009,35(11):2000-2007,8.基金项目
本研究由国家转基因生物新品种培育科技重大专项(2009ZX08001-005B,019),国家科技支撑计划重大项目(2006BAD02A03).江苏省自然科学基金项目(BK2009321),江苏省农业科技自主创新基金项目(CX[07]603),江苏省政府留学奖学金,江苏省农业科学院基金项(6110703,6510806)资助. （2009ZX08001-005B,019）
