作物学报2009，Vol.35Issue(11)：2064-2072,9.DOI:10.3724/SP.J.1006.2009.02064
栽培小麦Brock和京411感染白粉菌后蛋白质组的变化
Proteome Changes in Wheat Varieties Brock and Jing 411 after Inoculating Blumenia graminis
摘要
Abstract
The protein of resistant wheat (Triticum aestivum L.) variety Brock and susceptible variety Jing 411 was extracted from leaves and separated using two-dimensional polyacrylamid gel electrophoresis (2-DE) at 12 h, 3 d, and 5 d after inoculating prevalent race No. 15 of B. graminis f. sp. tritici. In Brock, compared with control with no pathogen inoculation, at least six pro-tein spots of 43 kD/pI 6.7, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8, 26 kD/pI 5.5 and 26 kD/pI 6.5 obviously increased in content at 12 h time point; and five protein spots of 48 kD/pI 5.6, 43 kD/pI 6.9, 43 kD/pI 7.2, 28 kD/pI 5.8 and 26 kD/pI 5.5 increased in content at 3 d time point. At 5d after inoculation, 12 novel proteins were induced, viz. 16 kD/pI 7.6, 42 kD/pI 6.5, 40 kD/pI 4.8, 40 kD/pI 4.6, 31 kD/pI 5.7, 16 kD/pI 4.6, 20 kD/pI 8.3, 50 kD/pI 6.7, 48 kD/pI 6.6, 28 kD/pI 5.7, 23 kD/pI 4.8 and 25 kD/pI 4.7; simultaneously, two protein spots that were observed earlier disappeared. In Jing 411, three protein spots of 21 kD/pI 6.4, 18 kD/pI 5.4, and 14 kD/pI 7.0 increased in content at 12 h after inoculation. At the 3 d time point, two protein spots of 80 kD/pI 5.4 and 14 kD/pI 7.0 increased in content, however, one protein spot (16 kD/pI 5.4) showed decrease in protein abundance. At the 5 d time point, three protein spots of 50 kD/pI 7.3, 40 kD/pI 7.3, and 24 kD/pI 7.2 and two protein spots of 40 kD/pI 4.8 and 14 kD/pI 7.2 showed increase and decrease in protein abundance, respectively, but there were no novel protein spots induced. Among the 12 novel protein spots induced in Brock, six spots were identified using MALDI-TOF-MS and NCB1 database searching, which were F-box and leucine-rich repeat protein, heavy metal transport/detoxification protein, endo-beta-l,3-glucanase (two isozymes), beta-1,3-glucanase precursor and zinc finger protein. These proteins are involved in a wide range of physiological processes, such as cell cycle control, development, phytohormone response, and resistance to fungal disease. Thus, the proteorne changes in Brock and Jing 411 leaves are probably associated with the resistance and susceptibility to powder mildew, respectively.关键词
白粉病/小麦/蛋白质组/β-1/3-葡聚糖酶/MALDI-TOF-MSKey words
Powdery mildew/Wheat/Proteome/Beta-1,3-glucanase/MALDI-TOF-MS分类
农业科技引用本文复制引用
于振,杨作民,李倩,赵建叶,江帆,王振英,彭永康,解超杰,刘志勇,孙其信..栽培小麦Brock和京411感染白粉菌后蛋白质组的变化[J].作物学报,2009,35(11):2064-2072,9.基金项目
本研究由国家自然科学基金项目(30771346),天津市科委重点项目(08JCZDJC16500),天津市教委重点项目(2006ZD08)资助. （30771346）
