作物学报2009，Vol.35Issue(11)：2107-2115,9.DOI:10.3724/SP.J.1006.2009.02107
1BL·1RS特异性分子标记的筛选及其对不同来源小麦品种1RS易位染色体的鉴定
Evaluation of Molecular Markers Specific for 1BL·1RS Translocation and Characterization of 1RS Chromosome in Wheat Varieties from Different Origins
摘要
Abstract
For the purpose of discriminating the 1BL·1RS translocation for wheat quality improvement, a total of ten STS, one SCAR and four RAPD markers specific to 1BL-1RS translocations as well as 22 SSR markers located on 1RS were employed to detect 78 accessions of 1BL·1RS translocations, 10 non- 1BL·IRS translocations, and three rye materials. The 1BL·1RS transloca-tions included Zhou 8425B and its 36 derivatives, Lankao 906 and its five derivatives, Aimengniu and its eight derivatives, and Lovrin series with 1BL·1RS transiocation (29 accessions). With the help of seven STS, one SCAR, three RAPD, and three SSR markers, the 1BL·1RS translocations were identified effectively. Markers ω-sec-p1/ω-sec-p2, ω-sec-p3/ω-sec-p4, H20, and SECA2/SECA3 were the best with stable amplification, clear bands on electrophoresis gel, good repeatability, and simple opera-tion. In the 78 1BL·1RS translocations, no diversities were observed according to the markers located on 1RS.关键词
小麦1BL·1RS易位系/STS标记:SCAR标记/RAPD标记/SSR标记Key words
1 BL·1RS translocation of wheat/STS marker/SCAR marker/RAPD marker/SSR marker分类
农业科技引用本文复制引用
唐怀君,殷贵鸿,夏先春,冯建军,曲延英,何中虎..1BL·1RS特异性分子标记的筛选及其对不同来源小麦品种1RS易位染色体的鉴定[J].作物学报,2009,35(11):2107-2115,9.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2006AA10Z1A7和2006AA100102)资助. （863计划）
