作物学报2009，Vol.35Issue(11)：2122-2126,5.DOI:10.3724/SP.J.1006.2009.02122
海南普通野生稻(Oryza rufipogon Griff.)Adh2基因多态性分析
Nucleotide Polymorphism in the Adh2 Gene of Oryza rufipogon Griff. from Hainan Island
摘要
Abstract
The sequence polymorphism of Adh2 in six populations of Oryza rufipogon (WN, WA, WC, DZ, QH, and LD) from Haman and two Oryza sativa (Nipponbare, 9311) was analyzed. The result showed that there were nine singleton variable sites, four parsimony informative sites, eight synonymous sites and five replacement sites at coding region. There were 21 singleton variable sites, 29 parsimony informative sites and 161 indels sites at intron region. Adh2 polymorphism (π_R, θ_R) at entire region was 0.011. The polymorphism at intron was higher than that at coding region. At coding region, the polymorphism of synonymous sites was higher than that at replacement sites. Neutrality tests showed that there was more purified selection at coding region than at intron region, although no significant difference was found. Cluster analysis showed O. rufipogon from Hainan could be di-vided into two groups. One group (QH, LD) was clustered with O. rufipogon from China mainland and O. sativa. The other group was clustered with some O. rufipogon from southeast Asia.关键词
普通野生稻/Adh2/序列多态性Key words
Oryza rufipogon/Adh2/Sequence polymorphism分类
农业科技引用本文复制引用
董轶博,裴新梧,袁潜华,彭于发..海南普通野生稻(Oryza rufipogon Griff.)Adh2基因多态性分析[J].作物学报,2009,35(11):2122-2126,5.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2007CB109202)和转基因生物新品种培育重大专项课题(2008ZX08011-001)资助. （973计划）
