作物学报2009，Vol.35Issue(12)：2139-2149,11.DOI:10.3724/SP.J.1006.2009.02139
大豆蛋白质有关性状的QTL定位
QTL Mapping of Protein Related Traits in Soybean [Glycine max (L.) Merr.]
摘要
Abstract
Soybean processing industry places emphasis on the quality of soybean protein which is related to the protein components, mainly 11S, 7S and 11S/7S, and their subunit constituents. In the improvement of soybean protein quality, the knowledge of genetic structure of the traits related to protein quality is of great importance. Therefore, the present paper was aimed at mapping QTLs of 16 traits, including protein content, protein plus fat content, fat content, 11S, 7S, 11S/7S, and subunit groups. Two populations, RIKY population with 184 recombinant inbred lines derived from Kefeng lxNannong 1138-2 and BIEX population with 114 BC_1F_2 lines derived from (Essex×ZDD2315)×ZDD2315, were used to map QTLs with the softwares of composite interval mapping (CIM), multiple interval mapping (MIM) of WinQTL Cartographer Ver. 2.5 and the inclusive composite interval mapping (ICIM) of IciMapping. The results showed that there were totally 17~+ QTLs detected with 11 for protein content, fat content and total content of protein and fat, and 1~+ and 3~+ for 11S subunit groups and 7S subunit groups, respectively, in RIKY, as well as totally 21~+ detected with only 2~+ for protein content, fat content and protein plus fat content, but 9~+ and 6~+ for 11S subunit groups and 7S subunit groups, respectively, in BIEX. There was no shared QTL detected for all 16 traits in both populations, indicating that the 16 traits between RIKY and BIEX have completely different genetic systems, and there existed obvious genetic differences between two parents of RIKY in protein content, fat content and protein plus fat content but less genetic differences in US subunit groups and 7S subunit groups, and those of BIEX were on the contrary. The group of protein content, fat content and protein plus fat content and the group of 11S, 7S and 11S/7S had common QTLs, showing their common genetic base, but there were no common QTLs in the groups of US subunit and 7S subunit. The results from QTL mapping and segregation analysis showed jointly that both major genes and minor genes contributed a large part of phenotypic variations for all 16 traits, suggesting that both major genes and minor genes should be considered in the breeding for protein-related traits.关键词
大豆/蛋白质含量/蛋油总量/11S/7S/11S/7S/亚基组/QTL定位Key words
Soybean/ Protein content/ Protein plus fat content/ 11S/ 7S/ 11S/7S/ Subunit group/ QTL mapping分类
农业科技引用本文复制引用
刘顺湖,周瑞宝,喻德跃,陈受宜,盖钧镒..大豆蛋白质有关性状的QTL定位[J].作物学报,2009,35(12):2139-2149,11.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2004CB7206,2006CB101708,2009CB118404),国家自然科学基金项目(30671266),国家高新技术研究发展计划(863计划)项目(2006AA100104),教育部高等学校创新引智计划项目(B08025)和农业部公益性行业专项(200803060)资助. （973计划）
