作物学报2009，Vol.35Issue(12)：2205-2212,8.DOI:10.3724/SP.J.1006.2009.02205
裸燕麦种质资源AFLP标记遗传多样性分析
Genetic Diversity in Naked Oat (Avena nuda) Germplasm Revealed by AFLP Markers
摘要
Abstract
Oat (Avena L.) is one of the most important cereal crops in the world, ranked at the sixth top place in planting area and yield among all cereal crops, and possesses high values in food and nutrition, health protection and feeding livestock. Naked oat (A. Nuda) is an endemic type in China. However, a few studies on naked oat germplasm at molecular level have been reported. The aim of this study was to evaluate the genetic diversity of core collection of naked oat using AFLP markers. A total of 281 accessions of naked oat were analyzed using 20 AFLP primer combinations. Selective amplification created 1 137 bands, of which 260 were polymorphic, accounting for 22.96% of the total bands. The mean polymorphism information content (PIC) was 0.0326. For different geographic groups, Simpson's index ranged from 1.235 to 1.495, and Shannon's index varied from 0.1558 to 0.4437. The majority (83.45%) of the AFLP variation resided within accessions of each group, and the rest (16.55%) existed among accessions between groups. The sample size of geographic groups was significantly associated with the number of polymorphic loci, proportion of within-group variation, Simpson's index and Shannon' s index. Accessions from Inner Mongolia and Shanxi were most diverse, and those from northeastern China were most distinct. Genetic resemblance was found within accessions from western China. Germplasm from East Europe was genetically close to that from Inner Mongolia, China. The genetic diversity of Chinese accessions was significantly higher than that of exotic accessions. Compared with breeding cultivars, landraces presented a higher proportion of within-group variation. Naked oat landraces were suggested to be collected in the regions where are not well represented by the current collections, and collecting activities should be continuous in the diversity-rich areas such as northwestern and southwestern China in order to enrich naked oat gene pool in China.关键词
裸燕麦/AFLP/遗传多样性/种质资源Key words
Avena nuda/ AFLP/ Genetic diversity/ Genetic resources分类
农业科技引用本文复制引用
徐微,张宗文,吴斌,崔林..裸燕麦种质资源AFLP标记遗传多样性分析[J].作物学报,2009,35(12):2205-2212,8.基金项目
本研究由国家科技支撑计划项目(2006BAD02B05-11)和国家燕麦产业技术体系建设项目资助. （2006BAD02B05-11）
