作物学报2009，Vol.35Issue(12)：2218-2224,7.DOI:10.3724/SP.J.1006.2009.02218
甘薯IbNPR1全长cDNA序列的分离与表达特性分析
Isolation and Characterization of IbNPR1 Gene from Sweet Potato (Ipomoea batatas)
摘要
Abstract
NPR1 (non-expressor of pathogenesis-related genes 1) protein is a key regulator of salicylic acid (SA)-mediated gene epression in systemic acquired resistance (SAR). By using homologous cloning and RACE (rapid amplification of cDNA ends) techniques, a full-length cDNA of IbNPR1 (Ipomoea batatas non-expressor of pathogenesis-related genes 1) was isolated from sweet potato var. Qingnong 2. The full length cDNA was 2 353 bp, including an ORF (open reading frame) putatively encoding a polypeptide of 586 amino acids residues with a predicted molecular mass of 64.851 kD. The deduced amino acid sequence shared structural features with known NPR1 (-like) proteins: ankyrin repeat and BTB/POZ. Furthermore, phylogenetic analysis showed IbNPR1 had the closest association with LeNPR1 from Lycopersicon esculentum. Southern-blot analysis revealed that the IbNPRl belonged to low-copy gene family in sweet potato. Semi-quantitative RT-PCR analysis indicated that IbNPRl was constitutively expressed in roots, stems and leaves. In addition, IbNPR1 could be induced by salicylic acid. The results suggest that IbNPRl plays an important role in the response to pathogen infections in sweet potato.关键词
甘薯/IbNPR1基因/抗病/cDNA末端快速扩增技术Key words
Ipomoea batatas/ IbNPR1 gene/ Disease resistance/ RACE分类
农业科技引用本文复制引用
陈观水,周以飞,林生,张铮,潘大仁..甘薯IbNPR1全长cDNA序列的分离与表达特性分析[J].作物学报,2009,35(12):2218-2224,7.基金项目
This study was supported by Natural Science Foundation of Fujian Province(2006J0059),National Natural Science Foundation of China(30900915),and the foundation for young scholars in Fujian Agriculture and Forestry University (08B12). （2006J0059）
