植物研究2009，Vol.29Issue(4)：492-499,8.
黄独脱毒苗叶片和茎段再生体系的建立
Establishment of Regeneration System of Leaves and Stems of Dioscorea bulbifera L. Virus-free Plantlets
摘要
Abstract
Effects of different factors on regeneration of leaves and stems were studied by using Dioscorea bulbifera L. as test material to optimize the regeneration system of leaves and stems of D. bulbifera L. virus-free plantlets. The results showed: The callus induction medium of D. bulbifera L. leaves and stems was MS + KT 2 mg · L~(-1) +2,4-D 2 mg · L~(-1); The best sucrose concentration of callus induction of D. bulbifera L. leaves and stems was 30 g · L~(-1) and 50 g · L~(-1) respectively; Calli of D. bulbifera L. leaves and stems were induced easily in dark; The differentiation medium of D. bulbifera L. leaf and stem calli was MS + KT 4 mg · L~(-1) + NAA 0. 1 mg · L ~(-1); The calli of D. bulbifera L. leaves and stems were differentiated easily after subcultured for two times; The best rooting medium of D. bulbifera L. adventitious buds was 1/2MS + IBA 0. 1 mg · L~(-1) + NAA 0. 5 mg · L ~(-1) + PP_(333)1 mg · L ~(-1). The regeneration system from leaves and stems of D. bulbifera L. virus-free plant-lets was established successfully for the first time, which providing a technological basis for factory production of D. bulbifera L. virus-free plantlets.关键词
黄独/脱毒苗/叶片/茎段/再生体系Key words
Dioscorea bulbifera L./ virus-free plantlets/ leaf/ stem/ regeneration system分类
农业科技引用本文复制引用
尹明华,洪森荣..黄独脱毒苗叶片和茎段再生体系的建立[J].植物研究,2009,29(4):492-499,8.基金项目
江西省教育厅科技项目(GJJ09374) （GJJ09374）
上饶师范学院2009～2010年度院级科技项目 （）
