中药材2009，Vol.32Issue(10)：1495-1499,5.
转基因何首乌毛状根生物转化麝香草酚
Biotransformation of Thymol by Hairy Roots of Transgenic Polygonum multiflorum
摘要
Abstract
Objective: The exogenous substrate, thymol, was firstly biotransformed by using suspension hairy roots of transgenic Polygonum midtiflorum, and its biotransformed situation was also investigated Methods: After five days co-cultivated period, the transformed product was isolated by Thin Layer Chromatograph and Column Chromatograph, with the structure elucidated by physic-chemical methods and spectra data. Meanwhile, the time course of biotransformation (T-C) for thymol was also measured by HPLC to illuminate its bio-transformed situation. Results; The glycosylated product, namely DMP, was isolated and purified, which structure was determined as 5-methyl-2-( l-methylethyl)phenyl-β-D-glucopyranoside. And the distribution of DMP in the medium or culture was varied in different co-cultivated periods, and for five days co-cultivated period, it mainly existed in the medium. Conclusion: The hairy roots of Polygonum midtiflorum were able to convert the aromatic exogenous substrate, thymol, into its glycoside. Furthermore, the time course indicated the relationship between DMP and co-cultivated period.关键词
何首乌/毛状根/生物转化/麝香草酚Key words
Polygonum midtiflorum Thunb./ Hairy roots/ Biotransformation/ Thymol分类
生物科学引用本文复制引用
董权锋,贾郡璋,朱建华,于荣敏..转基因何首乌毛状根生物转化麝香草酚[J].中药材,2009,32(10):1495-1499,5.基金项目
广东省自然科学基金(04010461) （04010461）
