中药材2009，Vol.32Issue(10)：1563-1567,5.
大豆苷元在Caco-2细胞模型中的转运和代谢
Transport and Metablism of Daidzein in Caco-2 Cell Model
摘要
Abstract
Objective: To investigate the intestinal absorption and metablism of daidzein in Caco-2 cell model. Methods: The damage of daidzein to Caco-2 cell was evaluated by MTT value and alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) activity. With the reference to propranolol, the apparent permeability coefficient (Papp) of daidzein was measured through the monolayer under the different concentrations and pH media. The metabolites were also measured by enzyme hydrolysis. Results: Daidzein had no damage to the growth of the Caco-2 cells in the concentration of 1 ～50 μg/mL The apparent permeability coefficient (Papp) of daidzein across the monolayer showed concentration- and pH- independent manner, which was similar to that of transcellcular marker-prop-ranolol, suggesting the good absorption of daidzein in vivo. By hydrolysis with β-glucuronidase, low metabolites were detected in monolayer and transport medium, verifying the existence of glucuronides and sulfates. Conclusion: The daidzein absorption in Caco-2 cell model is a passive and transcellular transport and quite good in the intestines.关键词
大豆苷元/Caco-2细胞模型/转运Key words
Daidzein/ Caco-2 cell model/ Transport分类
医药卫生引用本文复制引用
葛月宾,马燕,李卫中..大豆苷元在Caco-2细胞模型中的转运和代谢[J].中药材,2009,32(10):1563-1567,5.基金项目
湖北省自然科学基金面上项目(2007ABA357) （2007ABA357）
