中药材2009，Vol.32Issue(10)：1606-1609,4.
聚酰胺颗粒分离纯化土茯苓总黄酮研究
Separation and Purification of Total Flavonoids in Smilax glabra by Polyamide Resins
摘要
Abstract
Objective: To investigate the process of separating and purifying total flavonoids from Smilax glabra. Methods: With the contents of total flavonoids, astilbin and engelitin as indices, the optimum process of separating and purifying flavonoids from Smilax glabra was screened by static and dynamic adsorption tests. Results: The static saturated adsorption capacity of polyamide resin to flavonoids of Smilax glabra was 144 mg/g(saturated in 2 h). The dynamic adsorption capacity was 128. 125 mg/g, the optimum conditions of elution were as follows; the adsorbed resin was washed by 20BV water firstly, then by 20 BV 95% ethanol. The content of obtained total flavonoids reached 71. 84%, as the content of astilbin was 14. 32%, and the content of engelitin was 1. 08%. Conclusions : The total flavonoids of Smilax glabra is able to be easily separated and purified by polyamide resin under the optimum conditions above.关键词
土茯苓/总黄酮/落新妇苷/黄杞苷/聚酰胺Key words
Smilax glabra Roxb./ Total flavonoids/ Astilbin/ Engelitin/ Polyamide resin分类
医药卫生引用本文复制引用
吴新荣,刘志刚,颜仁梁,孙维峰..聚酰胺颗粒分离纯化土茯苓总黄酮研究[J].中药材,2009,32(10):1606-1609,4.基金项目
"十一五"军队中医药研发推广专项课题(2006062004) （2006062004）
