中国动脉硬化杂志2011，Vol.19Issue(10)：809-812,4.
氨氯地平对氧化型低密度脂蛋白诱导的人脐静脉内皮细胞CD40表达的影响
Effect of Amlodipine on CD40 Expression in HUVEC-12 Endothelial Cells Induced by Oxidized Low Density Lipoprotein
摘要
Abstract
Aim To study the effect of Amlodipine on CD40 expression in HUVEC-12 endothelial cells induced by oxidized low density lipoprotein ( ox-LOL) to explore the new antiatherosclerotic role of Amlodipine. Methods The experiment groups included 6 groups; control group, 10. 0 μmol/L Amlodipine alone treated group, ox-LOL group, and different concentration (0. 1, 1.0, 10. 0μml/L) of Amlodipine pretreated for 1 h and treated with 50 mg/L ox-LDL for 24 h group. RT-PCR was used to detect the mRNA expression of CD40 and western-blot was used to detect the protein expression of CD40. Results RT-PCR and western blot showed that with ox-LDL and Amlodipine treatment, bothmRNA and protein expression of CD40 were greatly decreased in a concentration-dependent manner. ConclusionAmlodipine can have a significant effect on down-regulating CD40 expression induced by ox-LDL.关键词
氨氯地平/氧化型低密度脂蛋白/HUVEC-12内皮细胞/白细胞分化抗原40Key words
Amlodipine/Oxidized Low Density Upoprotein/HUVEC-12 Endothelial Cells/CD40分类
医药卫生引用本文复制引用
涂光辉,尹凯,周志刚,孟军,熊紫薇,涂剑,罗招阳..氨氯地平对氧化型低密度脂蛋白诱导的人脐静脉内皮细胞CD40表达的影响[J].中国动脉硬化杂志,2011,19(10):809-812,4.基金项目
湖南省卫生厅科技项目(B2007092,2007101),衡阳市科学技术发展项目(2010kj17) （B2007092,2007101）
